Overview
- External ontology
- MEDIADB:72 (source link unavailable) — BGDM
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007420["bgdm"]
ing0_L_Glutamate["L-Glutamate"]
CultureMech_007420 --> ing0_L_Glutamate
CHEBI_16015["CHEBI:16015"]
ing0_L_Glutamate -.-> CHEBI_16015
ing1_Alanine["Alanine"]
CultureMech_007420 --> ing1_Alanine
CHEBI_16977["CHEBI:16977"]
ing1_Alanine -.-> CHEBI_16977
ing2_Phenylalanine["Phenylalanine"]
CultureMech_007420 --> ing2_Phenylalanine
CHEBI_17295["CHEBI:17295"]
ing2_Phenylalanine -.-> CHEBI_17295
ing3_Menadione["Menadione"]
CultureMech_007420 --> ing3_Menadione
CHEBI_28869["CHEBI:28869"]
ing3_Menadione -.-> CHEBI_28869
ing4_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007420 --> ing4_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing4_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing5_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007420 --> ing5_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing5_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing6_Aspartate["Aspartate"]
CultureMech_007420 --> ing6_Aspartate
CHEBI_29995["CHEBI:29995"]
ing6_Aspartate -.-> CHEBI_29995
ing7_Sodium_chloride["Sodium chloride"]
CultureMech_007420 --> ing7_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing7_Sodium_chloride -.-> CHEBI_26710
ing8_Cysteine_HCl["Cysteine-HCl"]
CultureMech_007420 --> ing8_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing8_Cysteine_HCl -.-> CHEBI_91247
ing9_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007420 --> ing9_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing9_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing10_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007420 --> ing10_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing10_Magnesium_sulfate -.-> CHEBI_32599
ing11_Hemin["Hemin"]
CultureMech_007420 --> ing11_Hemin
CHEBI_50385["CHEBI:50385"]
ing11_Hemin -.-> CHEBI_50385
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Glutamate
C5H9NO4 147.13 g/mol
|
0.6797 MILLIMOLAR |
CHEBI:16015
L-glutamic acid
|
Cross-references: KEGG:glu-L |
| 2 |
Alanine
C3H7NO2 89.094 g/mol
|
1.1224 MILLIMOLAR |
CHEBI:16977
L-alanine
|
|
| 3 |
Phenylalanine
C9H11NO2 165.192 g/mol
|
0.6054 MILLIMOLAR |
CHEBI:17295
L-phenylalanine
|
|
| 4 |
Menadione
C11H8O2 172.183 g/mol
|
0.002904 MILLIMOLAR |
CHEBI:28869
menadione
|
Cross-references: KEGG:mndn |
| 5 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.9011 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 6 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
1.1481 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
Aspartate
C4H5NO4 131.087 g/mol
|
0.7513 MILLIMOLAR |
CHEBI:29995
aspartate(2-)
|
|
| 8 |
Sodium chloride
Cl.Na 58.443 g/mol
|
1.7112 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 9 |
Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.4758 MILLIMOLAR |
CHEBI:91247
L-cysteine hydrochloride
|
|
| 10 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
7.3483 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 11 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
2.8401 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 12 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
0.007669 MILLIMOLAR |
CHEBI:50385
hemin
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.270851Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 72, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:48.970120Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007420 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:07.346377+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BGDM" to "bgdm" for consistent matching |
| 2026-06-05T07:40:50.854120+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-20T19:01:34.633477+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |