Overview
- External ontology
- MEDIADB:79 (source link unavailable) — M9 minimal medium with l-malate
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007427["m9_minimal_medium_with_l_malate"]
ing0__S__Malate["(S)-Malate"]
CultureMech_007427 --> ing0__S__Malate
ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007427 --> ing1_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing2_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
CultureMech_007427 --> ing2_Dibasic_sodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing2_Dibasic_sodium_phosphate -.-> CHEBI_34683
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_007427 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_007427 --> ing4_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing4_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing5_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007427 --> ing5_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing5_Magnesium_sulfate -.-> CHEBI_32599
ing6_Ammonium_chloride["Ammonium chloride"]
CultureMech_007427 --> ing6_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing6_Ammonium_chloride -.-> CHEBI_31206
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | (S)-Malate | 15.0 MILLIMOLAR | Cross-references: KEGG:mal-L | |
| 2 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.1 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 3 |
Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
|
49.0 MILLIMOLAR |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
|
8.6 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 5 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
22.0 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
2.0 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 7 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
19.0 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.336155Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 79, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:49.001249Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007427 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:07.379948+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M9 minimal medium with l-malate" to "m9_minimal_medium_with_l_malate" for consistent matching |
| 2026-06-05T07:41:12.052257+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-31T04:06:25.305988+00:00 | repair_mediadb_names.py | REPAIRED_MEDIADB_TRUNCATED_NAME | Restored 1 ingredient name(s) truncated at a parenthesis by the MediaDB SQL parser. Resolved against MEDIADB:79's own compound list in media_database.07Oct2015.sql, requiring a unique prefix match and, where more than one matched, an equal Amount_mM. |