p2_1_5_butanol_challenge

CultureMech:007439 bacterial DEFINED LIQUID
Original name: P2, 1.5% butanol challenge

Overview

External ontology
MEDIADB:91 (source link unavailable) — P2, 1.5% butanol challenge
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007439["p2_1_5_butanol_challenge"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_007439 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_Biotin["Biotin"]
    CultureMech_007439 --> ing1_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing1_Biotin -.-> CHEBI_15956
    ing2_Thiamine["Thiamine"]
    CultureMech_007439 --> ing2_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing2_Thiamine -.-> CHEBI_18385
    ing3_4_Aminobenzoate["4-Aminobenzoate"]
    CultureMech_007439 --> ing3_4_Aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing3_4_Aminobenzoate -.-> CHEBI_17836
    ing4_1_Butanol["1-Butanol"]
    CultureMech_007439 --> ing4_1_Butanol
    ing5_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_007439 --> ing5_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing5_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing6_Sodium_chloride["Sodium chloride"]
    CultureMech_007439 --> ing6_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing6_Sodium_chloride -.-> CHEBI_26710
    ing7_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007439 --> ing7_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing7_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing8_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007439 --> ing8_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing8_Magnesium_sulfate -.-> CHEBI_32599
    ing9_Manganese_sulfate["Manganese sulfate"]
    CultureMech_007439 --> ing9_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing9_Manganese_sulfate -.-> CHEBI_86364
    ing10_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_007439 --> ing10_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing10_Ferrous_sulfate -.-> CHEBI_75832
    ing11_Ammonium_Acetate["Ammonium Acetate"]
    CultureMech_007439 --> ing11_Ammonium_Acetate
    CHEBI_62947["CHEBI:62947"]
    ing11_Ammonium_Acetate -.-> CHEBI_62947

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 D-Glucose
C6H12O6 180.156 g/mol
123.21 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
2 Biotin
C10H16N2O3S 244.316 g/mol
4.5429e-05 MILLIMOLAR CHEBI:15956
biotin
 Cross-references: KEGG:btn
3 Thiamine
C12H17N4OS 265.362 g/mol
0.00368963 MILLIMOLAR CHEBI:18385
thiamine(1+)
 Cross-references: KEGG:thm
4 4-Aminobenzoate
C7H6NO2 136.13 g/mol
0.00809302 MILLIMOLAR CHEBI:17836
4-aminobenzoate
 Cross-references: KEGG:4abz
5 1-Butanol 147.695 MILLIMOLAR
6 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
3.18564 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
7 Sodium chloride
Cl.Na 58.443 g/mol
0.189918 MILLIMOLAR CHEBI:26710
sodium chloride
8 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
4.07785 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
9 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.900619 MILLIMOLAR CHEBI:32599
magnesium sulfate
10 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.0656655 MILLIMOLAR CHEBI:86364
manganese(II) sulfate monohydrate
11 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.0399165 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
12 Ammonium Acetate
C2H3O2.H4N 77.083 g/mol
31.6775 MILLIMOLAR CHEBI:62947
ammonium acetate

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:43.421560Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 91, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:49.069646Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007439
2026-03-17T06:30:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:07.454251+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "P2, 1.5% butanol challenge" to "p2_1_5_butanol_challenge" for consistent matching
2026-06-05T07:41:40.730272+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.