Overview
- External ontology
- MEDIADB:92 (source link unavailable) — CDM; McNeil et al
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007440["cdm_mcneil_et_al"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007440 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Biotin["Biotin"]
CultureMech_007440 --> ing1_Biotin
CHEBI_15956["CHEBI:15956"]
ing1_Biotin -.-> CHEBI_15956
ing2_4_Aminobenzoate["4-Aminobenzoate"]
CultureMech_007440 --> ing2_4_Aminobenzoate
CHEBI_17836["CHEBI:17836"]
ing2_4_Aminobenzoate -.-> CHEBI_17836
ing3_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
CultureMech_007440 --> ing3_Calcium_chloride_anhydrous
CHEBI_3312["CHEBI:3312"]
ing3_Calcium_chloride_anhydrous -.-> CHEBI_3312
ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007440 --> ing4_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing5_Sodium_molybdate["Sodium molybdate"]
CultureMech_007440 --> ing5_Sodium_molybdate
CHEBI_75215["CHEBI:75215"]
ing5_Sodium_molybdate -.-> CHEBI_75215
ing6_Sodium_chloride["Sodium chloride"]
CultureMech_007440 --> ing6_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing6_Sodium_chloride -.-> CHEBI_26710
ing7_Thiamine_HCl["Thiamine HCl"]
CultureMech_007440 --> ing7_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing7_Thiamine_HCl -.-> CHEBI_49105
ing8_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007440 --> ing8_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing8_Magnesium_sulfate -.-> CHEBI_32599
ing9_Manganese_sulfate["Manganese sulfate"]
CultureMech_007440 --> ing9_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing9_Manganese_sulfate -.-> CHEBI_86364
ing10_Ferrous_sulfate["Ferrous sulfate"]
CultureMech_007440 --> ing10_Ferrous_sulfate
CHEBI_75832["CHEBI:75832"]
ing10_Ferrous_sulfate -.-> CHEBI_75832
ing11_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_007440 --> ing11_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing11_Ammonium_sulfate -.-> CHEBI_62946
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
166.611 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.004093 MILLIMOLAR |
CHEBI:15956
biotin
|
Cross-references: KEGG:btn |
| 3 |
4-Aminobenzoate
C7H6NO2 136.13 g/mol
|
0.01458 MILLIMOLAR |
CHEBI:17836
4-aminobenzoate
|
Cross-references: KEGG:4abz |
| 4 |
Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
|
0.04564 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
|
| 5 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
5.7405 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Sodium molybdate
MoO4.2Na 205.916 g/mol
|
0.04856 MILLIMOLAR |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 7 |
Sodium chloride
Cl.Na 58.443 g/mol
|
1.711 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
|
| 8 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.00593 MILLIMOLAR |
CHEBI:49105
thiamine hydrochloride
|
|
| 9 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.40572 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 10 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.06724 MILLIMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 11 |
Ferrous sulfate
Fe.O4S 151.908 g/mol
|
0.09874 MILLIMOLAR |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 12 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
15.135 MILLIMOLAR |
CHEBI:62946
ammonium sulfate
|
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.430126Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 92, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:49.076093Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007440 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:07.461435+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CDM; McNeil et al" to "cdm_mcneil_et_al" for consistent matching |
| 2026-06-05T07:40:52.821009+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |