m9_with_0_4_glucose_and_20_emulsion

CultureMech:007445 bacterial DEFINED LIQUID
Original name: M9 with 0.4% glucose and 20% emulsion

Overview

External ontology
MEDIADB:97 (source link unavailable) — M9 with 0.4% glucose and 20% emulsion
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007445["m9_with_0_4_glucose_and_20_emulsion"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_007445 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007445 --> ing1_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing2_Tween_80["Tween 80"]
    CultureMech_007445 --> ing2_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing2_Tween_80 -.-> CHEBI_53426
    ing3_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007445 --> ing3_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing3_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing4_Sodium_chloride["Sodium chloride"]
    CultureMech_007445 --> ing4_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing4_Sodium_chloride -.-> CHEBI_26710
    ing5_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007445 --> ing5_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing5_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing6_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007445 --> ing6_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing6_Magnesium_sulfate -.-> CHEBI_32599
    ing7_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007445 --> ing7_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing7_Ammonium_chloride -.-> CHEBI_31206
    ing8_Hexadecane["Hexadecane"]
    CultureMech_007445 --> ing8_Hexadecane
    CHEBI_45296["CHEBI:45296"]
    ing8_Hexadecane -.-> CHEBI_45296

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 D-Glucose
C6H12O6 180.156 g/mol
22.2024 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
2 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.1 MILLIMOLAR CHEBI:3312
calcium dichloride
3 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
12.219 MILLIMOLAR CHEBI:53426
polysorbate 80
4 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
47.743 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
5 Sodium chloride
Cl.Na 58.443 g/mol
8.5557 MILLIMOLAR CHEBI:26710
sodium chloride
6 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
22.0448 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
7 Magnesium sulfate
Mg.O4S 120.368 g/mol
2.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
8 Ammonium chloride
Cl.H4N 53.492 g/mol
18.6947 MILLIMOLAR CHEBI:31206
ammonium chloride
9 Hexadecane
C16H34 226.448 g/mol
883.236 MILLIMOLAR CHEBI:45296
hexadecane

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:43.479948Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 97, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:49.101949Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007445
2026-03-17T06:30:34.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:07.490416+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M9 with 0.4% glucose and 20% emulsion" to "m9_with_0_4_glucose_and_20_emulsion" for consistent matching
2026-06-05T07:41:12.115011+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.