m9_with_1_0_glucose_prachaiyo_et_al

CultureMech:007447 bacterial DEFINED LIQUID
Original name: M9 with 1.0% glucose (prachaiyo et al)

Overview

External ontology
MEDIADB:99 (source link unavailable) — M9 with 1.0% glucose (prachaiyo et al)
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_007447["m9_with_1_0_glucose_prachaiyo_et_al"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_007447 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_007447 --> ing1_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing1_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing2_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_007447 --> ing2_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing2_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_007447 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_007447 --> ing4_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing4_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing5_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_007447 --> ing5_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing5_Magnesium_sulfate -.-> CHEBI_32599
    ing6_Ammonium_chloride["Ammonium chloride"]
    CultureMech_007447 --> ing6_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing6_Ammonium_chloride -.-> CHEBI_31206

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 D-Glucose
C6H12O6 180.156 g/mol
55.5062 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
2 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.1 MILLIMOLAR CHEBI:3312
calcium dichloride
3 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
47.743 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
4 Sodium chloride
Cl.Na 58.443 g/mol
8.5557 MILLIMOLAR CHEBI:26710
sodium chloride
5 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
22.0448 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
6 Magnesium sulfate
Mg.O4S 120.368 g/mol
2.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
7 Ammonium chloride
Cl.H4N 53.492 g/mol
18.6947 MILLIMOLAR CHEBI:31206
ammonium chloride

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:43.500608Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 99, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:49.111321Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007447
2026-03-17T06:30:36.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:07.500574+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'M9 with 1.0% glucose (prachaiyo et al" to "m9_with_1_0_glucose_prachaiyo_et_al" for consistent matching
2026-06-05T07:41:12.200692+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T06:17:36.659230+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.