Acidimicrobium Medium (CultureMech:007449)

Overview

External ontology: nbrc.medium:1002 (source link unavailable) — NBRC Medium 1002
pH: 2.0
Notes: Source: NBRC Medium 1002 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1002 NBRC lists Acidimicrobium Medium with MgSO4·7H2O, (NH4)2SO4, K2HPO4, KCl, and distilled water, adjusts the base medium to pH 2.0 with H2SO4, and provides heterotrophic yeast-extract and autotrophic ferrous-sulfate growth modes.

Composition

flowchart LR
    CultureMech_007449["Acidimicrobium Medium"]
    ing0_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007449 --> ing0_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_7H2O -.-> CHEBI_31795
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007449 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_K2HPO4["K2HPO4"]
    CultureMech_007449 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_KCl["KCl"]
    CultureMech_007449 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Distilled_water["Distilled water"]
    CultureMech_007449 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_H2SO4["H2SO4"]
    CultureMech_007449 --> ing5_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing5_H2SO4 -.-> CHEBI_26836
    ing6_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_007449 --> ing6_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_7H2O -.-> CHEBI_75836
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_007449 --> ing7_Yeast_extract

Ingredients (8)

#	Ingredient	Concentration	Ontology	Notes
1	MgSO4·7H2O 7H2O.Mg.O4S 246.473 g/mol	0.5 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	NBRC Medium 1002 lists 0.5 g MgSO4·7H2O.
2	(NH4)2SO4 2H4N.O4S 132.141 g/mol	0.4 G_PER_L	CHEBI:62946 ammonium sulfate	NBRC Medium 1002 lists 0.4 g (NH4)2SO4.
3	K2HPO4 HO4P.2K 174.174 g/mol	0.2 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	NBRC Medium 1002 lists 0.2 g K2HPO4.
4	KCl Cl.K 74.551 g/mol	0.1 G_PER_L	CHEBI:32588 potassium chloride	NBRC Medium 1002 lists 0.1 g KCl.
5	Distilled water H2O 18.015 g/mol	1 L	CHEBI:15377 water	NBRC Medium 1002 lists 1 L distilled water.
6	H2SO4 H2O4S 98.079 g/mol	variable VARIABLE	CHEBI:26836 sulfuric acid	NBRC Medium 1002 adjusts the base medium to pH 2.0 with H2SO4 and the autotrophic ferrous sulfate variant to pH 1.7 with H2SO4.
7	FeSO4·7H2O Fe.7H2O.O4S 278.013 g/mol	variable VARIABLE	CHEBI:75836 iron(2+) sulfate heptahydrate	NBRC Medium 1002 lists 10 mg/L FeSO4·7H2O for heterotrophic growth on yeast extract and 13.9 g/L FeSO4·7H2O for autotrophic growth on ferrous sulfate.
8	Yeast extract	0.25 G_PER_L	FOODON:03315426 yeast extract	NBRC Medium 1002 adds yeast extract from a sterile stock solution to 0.25 g/L final concentration for heterotrophic growth.

Preparation

Dissolve MgSO4·7H2O, (NH4)2SO4, K2HPO4, and KCl in 1 L distilled water.
Adjust the base medium to pH 2.0 with H2SO4.
For heterotrophic growth on yeast extract, add 10 mg/L FeSO4·7H2O to the medium.
Autoclave the medium before adding sterile yeast extract.
For heterotrophic growth on yeast extract, add sterile yeast extract stock solution after autoclaving to 0.25 g/L final concentration.
For autotrophic growth on ferrous sulfate, add 13.9 g/L FeSO4·7H2O and adjust medium pH to 1.7 with H2SO4 prior to autoclaving.

Target organisms

No target organisms are recorded.

Recipe variants

Name
nbrc_1002_heterotrophic_yeast_extract
Relationship
SUPPLEMENTED_VARIANT
Description
Heterotrophic NBRC Medium 1002 condition supplemented with ferrous sulfate heptahydrate and post-autoclave yeast extract.
Modifications
- Add 10 mg/L FeSO4·7H2O to the medium.
- After autoclaving, add yeast extract from a sterile stock solution to 0.25 g/L final concentration.
Purpose
Heterotrophic growth on yeast extract.
Evidence
- Reference
  https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1002
  Supports
  SUPPORT
  Explanation
  The NBRC Medium 1002 catalogue entry lists this separate heterotrophic growth condition.
Name
nbrc_1002_autotrophic_ferrous_sulfate
Relationship
SUPPLEMENTED_VARIANT
Description
Autotrophic NBRC Medium 1002 condition supplemented with a high ferrous sulfate heptahydrate concentration and acidified to pH 1.7.
Modifications
- Add 13.9 g/L FeSO4·7H2O to the medium.
- Adjust medium pH to 1.7 with H2SO4 prior to autoclaving.
Purpose
Autotrophic growth on ferrous sulfate.
Evidence
- Reference
  https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1002
  Supports
  SUPPORT
  Explanation
  The NBRC Medium 1002 catalogue entry lists this separate autotrophic growth condition.

Provenance

Timestamp	Curator	Action	Notes
2026-02-20T00:22:36.004823Z	nbrc-import	Imported from NBRC	Source: NBRC, Media No. 1002, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1002. Imported via ethical web scraping.
2026-03-09T08:05:11.769433+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T03:09:43.609831Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 9 duplicate ingredient(s)
2026-03-13T06:19:49.119547Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007449
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-05-16T09:03:06.550789+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-08-19T21:54:22.511329+00:00	recover_nbrc_composition.py	Recovered composition from source HTML	Re-parsed the NBRC medium 1002 composition table from the preserved scrape; 5 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 1 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.691661+00:00	apply_mim_groundings.py	Adopted MIM published grounding	Grounded 4 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-11T00:00:00-07:00	repair_nbrc_1003_score15.py	RESOLVED_NBRC_1003_SCORE15	Added the NBRC Medium 1002 source term, grounded the recovered Acidimicrobium Medium formula, and converted its pH, heterotrophic, and autotrophic source prose into structured preparation and variant fields.