1/10 Tryptic Soy Medium w/o Dextrose (pH 5.0)

CultureMech:007451 bacterial COMPLEX SOLID_AGAR

Overview

External ontology
nbrc.medium:1035 (source link unavailable) — NBRC Medium 1035
pH
5.0
Notes
Source: NBRC Medium 1035 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1035 NBRC lists 1/10 Tryptic Soy Medium without dextrose at pH 5.0, prepared from Bacto Tryptic Soy Broth without dextrose, NaCl, K2HPO4, optional agar, and distilled water.

Composition

flowchart LR
    CultureMech_007451["1/10 Tryptic Soy Medium w/o Dextrose (pH 5.0)"]
    ing0_Bacto_Tryptic_Soy_Broth_w_o_Dextrose__Difco_["Bacto Tryptic Soy Broth w/o Dextrose (Difco)"]
    CultureMech_007451 --> ing0_Bacto_Tryptic_Soy_Broth_w_o_Dextrose__Difco_
    ing1_NaCl["NaCl"]
    CultureMech_007451 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_K2HPO4["K2HPO4"]
    CultureMech_007451 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Agar__if_needed_["Agar (if needed)"]
    CultureMech_007451 --> ing3_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar__if_needed_ -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_007451 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Bacto Tryptic Soy Broth w/o Dextrose (Difco) 2.75 G_PER_L NBRC Medium 1035 lists 2.75 g Bacto Tryptic Soy Broth without dextrose.
2 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
 NBRC Medium 1035 lists 5 g NaCl.
3 K2HPO4
HO4P.2K 174.174 g/mol
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 NBRC Medium 1035 lists 2.5 g K2HPO4.
4 Agar (if needed) 15 G_PER_L CHEBI:2509
agar
 NBRC Medium 1035 lists 15 g agar if needed.
5 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
 NBRC Medium 1035 lists 1 L distilled water.

Preparation

  1. Dissolve Bacto Tryptic Soy Broth without dextrose, NaCl, K2HPO4, and agar if needed in 1 L distilled water.
  2. Adjust pH to 5.0.
  3. Sterilize by autoclaving.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:36.010533Z nbrc-import Imported from NBRC Source: NBRC, Media No. 1035, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1035. Imported via ethical web scraping.
2026-03-13T03:09:32.652995Z data-quality-cleanup-v1.0 Data quality fixes Merged 5 duplicate ingredient(s)
2026-03-13T06:19:49.125937Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007451
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Bacto Tryptic Soy Broth w/o Dextrose (Difco)2.75gNaCl5gK2HPO42.5gAgar (if needed)15gDistilled water1LpH 5.0
2026-05-16T09:03:06.560436+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.537307+00:00 recover_nbrc_composition.py Removed corrupt rows, kept curated composition Record already held a grounded ingredient list, so the NBRC medium 1035 page was NOT used — it lists only the commercial premix. Dropped the empty-name row and 0 unparsed solution blob(s) (#299).
2026-09-11T00:00:00-07:00 repair_nbrc_1038_score15.py RESOLVED_NBRC_1038_SCORE15 Added the NBRC Medium 1035 source term, replaced the expanded Tryptic Soy Agar approximation with the official commercial-premix formula, and recorded pH 5.0.