1051

Overview

External ontology

nbrc.medium:1050 (source link unavailable) — NBRC Medium 1050

pH

7.0

Notes

Source: NBRC Medium 1050 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1050 The NBRC A medium table lists 18 top-level rows, nested trace-elements and vitamin solutions, and a final pH of 7.0.

Composition

flowchart LR
    CultureMech_007452["1051"]
    ing0_NaCl["NaCl"]
    CultureMech_007452 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_007452 --> ing1_Bacto_Yeast_Extract__Difco_
    ing2_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_007452 --> ing2_MgCl2_6H2O
    ing3_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007452 --> ing3_MgSO4_7H2O
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007452 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_007452 --> ing5_CaCl2_2H2O
    ing6_KH2PO4["KH2PO4"]
    CultureMech_007452 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_KCl["KCl"]
    CultureMech_007452 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_NaBr["NaBr"]
    CultureMech_007452 --> ing8_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing8_NaBr -.-> CHEBI_63004
    ing9_H3BO3["H3BO3"]
    CultureMech_007452 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_SrCl2_6H2O["SrCl2·6H2O"]
    CultureMech_007452 --> ing10_SrCl2_6H2O
    ing11_Fe_III__ammonium_citrate["Fe(III) ammonium citrate"]
    CultureMech_007452 --> ing11_Fe_III__ammonium_citrate
    ing12_Bis_Tris_Propane["Bis Tris Propane"]
    CultureMech_007452 --> ing12_Bis_Tris_Propane
    ing13_Trace_elements_solution_["Trace elements solution*"]
    CultureMech_007452 --> ing13_Trace_elements_solution_
    ing14_Vitamin_solution__["Vitamin solution**"]
    CultureMech_007452 --> ing14_Vitamin_solution__
    ing15_Resazurin["Resazurin"]
    CultureMech_007452 --> ing15_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing15_Resazurin -.-> CHEBI_8806
    ing16_Na2S_9H2O["Na2S·9H2O"]
    CultureMech_007452 --> ing16_Na2S_9H2O
    ing17_Distilled_water["Distilled water"]
    CultureMech_007452 --> ing17_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing17_Distilled_water -.-> CHEBI_15377

Ingredients (18)

Preparation

1. Mix all ingredients except vitamin solution and Na2S·9H2O.
2. Adjust pH to 7.0 with H2SO4.
3. Autoclave the medium under a N2 atmosphere.
4. Autoclave Na2S·9H2O separately as a 5% solution under N2.
5. Filter-sterilize the vitamin solution.
6. Anaerobically add filter-sterile vitamin solution and sterile Na2S·9H2O.

Solutions

Target organisms

No target organisms are recorded.

Provenance