1075

CultureMech:007454 bacterial COMPLEX SOLID_AGAR

Overview

External ontology
nbrc.medium:1074 (source link unavailable) — NBRC Medium 1074
Notes
Source: NBRC Medium 1074 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1074 The NBRC Alkaline Agar table lists glucose, Bacto Peptone, Bacto Yeast Extract, KH2PO4, MgSO4·7H2O, 20% NaCl solution, 10% Na2CO3 solution, agar, and distilled water.

Composition

flowchart LR
    CultureMech_007454["1075"]
    ing0_Glucose["Glucose"]
    CultureMech_007454 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Bacto_Peptone__Difco_["Bacto Peptone (Difco)"]
    CultureMech_007454 --> ing1_Bacto_Peptone__Difco_
    ing2_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_007454 --> ing2_Bacto_Yeast_Extract__Difco_
    ing3_KH2PO4["KH2PO4"]
    CultureMech_007454 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007454 --> ing4_MgSO4_7H2O
    ing5_20__NaCl_solution["20% NaCl solution"]
    CultureMech_007454 --> ing5_20__NaCl_solution
    ing6_10__Na2CO3_solution["10% Na2CO3 solution"]
    CultureMech_007454 --> ing6_10__Na2CO3_solution
    ing7_Agar["Agar"]
    CultureMech_007454 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Distilled_water["Distilled water"]
    CultureMech_007454 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Glucose
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:17234
glucose
2 Bacto Peptone (Difco) 5 G_PER_L MICRO:0000178 (source link unavailable)
Bacto peptone
3 Bacto Yeast Extract (Difco) 5 G_PER_L FOODON:03315426
Yeast extract
4 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 MgSO4·7H2O 0.2 G_PER_L
6 20% NaCl solution 200 ML_PER_L
7 10% Na2CO3 solution 100 ML_PER_L
8 Agar 15 G_PER_L CHEBI:2509
agar
9 Distilled water
H2O 18.015 g/mol
700 ML_PER_L CHEBI:15377
water

Preparation

  1. Autoclave the 20% NaCl solution separately.
  2. Autoclave the 10% Na2CO3 solution separately.
  3. Aseptically add the NaCl and Na2CO3 solutions to the medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:36.017019Z nbrc-import Imported from NBRC Source: NBRC, Media No. 1074, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1074. Imported via ethical web scraping.
2026-03-09T08:05:03.763180+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:09:35.699282Z data-quality-cleanup-v1.0 Data quality fixes Merged 9 duplicate ingredient(s)
2026-03-13T06:19:49.134758Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007454
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-05-16T09:03:06.572410+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.568763+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 1074 composition table from the preserved scrape; 9 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 1 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.736797+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 4 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-07T00:00:00-07:00 repair_nbrc_partial_score25.py RESOLVED_NBRC_PARTIAL_SCORE25 Preserved the recovered NBRC Medium 1074 composition table.