1107

Overview

External ontology

nbrc.medium:1106 (source link unavailable) — NBRC Medium 1106

pH

7.0

Notes

Source: NBRC Medium 1106 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1106 The NBRC 1a Medium table lists pH 7.0 and instructs sealed-vessel autoclaving followed by syringe pH adjustment and addition of filter-sterilized methane before inoculation.

Composition

flowchart LR
    CultureMech_007456["1107"]
    ing0_NaNO3["NaNO3"]
    CultureMech_007456 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007456 --> ing1_MgSO4_7H2O
    ing2_Na2HPO4_2H2O["Na2HPO4·2H2O"]
    CultureMech_007456 --> ing2_Na2HPO4_2H2O
    ing3_KH2PO4["KH2PO4"]
    CultureMech_007456 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_CaCl2_6H2O["CaCl2·6H2O"]
    CultureMech_007456 --> ing4_CaCl2_6H2O
    ing5_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_007456 --> ing5_FeSO4_7H2O
    ing6_Trace_metal_solution_["Trace metal solution*"]
    CultureMech_007456 --> ing6_Trace_metal_solution_
    ing7_Distilled_water["Distilled water"]
    CultureMech_007456 --> ing7_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing7_Distilled_water -.-> CHEBI_15377

Ingredients (8)

Preparation

1. Dispense the medium into culture vessels and seal with butyl rubber stoppers.
2. Autoclave at 121 C for 15 minutes.
3. Cool to room temperature.
4. Adjust pH to 7.0 with sterile pH-adjusting agent by syringe without opening the vessel.
5. Filter-sterilize high-purity methane gas.
6. Before inoculation, aseptically add methane to an approximately 1:4 methane-to-air headspace.

Solutions

Target organisms

No target organisms are recorded.

Provenance