Overview
- External ontology
- nbrc.medium:1155 (source link unavailable) — NBRC Medium 1155
- Notes
- Source: NBRC Medium 1155 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1155 NBRC lists TG agar with Tryptone (Diffico), glucose, agar, and distilled water.
Composition
flowchart LR
CultureMech_007459["TG agar"]
ing0_Tryptone__Diffico_["Tryptone (Diffico)"]
CultureMech_007459 --> ing0_Tryptone__Diffico_
ing1_Glucose["Glucose"]
CultureMech_007459 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_Agar["Agar"]
CultureMech_007459 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Distilled_water["Distilled water"]
CultureMech_007459 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone (Diffico) | 10 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
NBRC Medium 1155 lists 10 g Tryptone (Diffico). |
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
3 G_PER_L |
CHEBI:17234
glucose
|
NBRC Medium 1155 lists 3 g glucose. |
| 3 | Agar | 10 G_PER_L |
CHEBI:2509
agar
|
NBRC Medium 1155 lists 10 g agar. |
| 4 |
Distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
NBRC Medium 1155 lists 1 L distilled water. |
Preparation
- Dissolve Tryptone (Diffico), glucose, and agar in 1 L distilled water.
- Sterilize by autoclaving.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-02-20T00:22:36.028725Z | nbrc-import | Imported from NBRC | Source: NBRC, Media No. 1155, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1155. Imported via ethical web scraping. |
| 2026-03-13T03:09:07.858555Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:49.150441Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007459 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Tryptone (Diffico)10gGlucose3gAgar10gDistilled water1L; Added default concentration for ingredient: |
| 2026-05-16T09:03:06.587850+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-08-19T21:54:22.617114+00:00 | recover_nbrc_composition.py | Recovered composition from source HTML | Re-parsed the NBRC medium 1155 composition table from the preserved scrape; 4 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299). |
| 2026-08-20T19:01:15.777203+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 3 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-09-11T00:00:00-07:00 | repair_nbrc_1158_score15.py | RESOLVED_NBRC_1158_SCORE15 | Added the NBRC Medium 1155 source term and official title, grounded all recovered TG agar ingredients, and converted the placeholder preparation into source-specific autoclave steps. |