Medium for the 2µ Mapping Strains

CultureMech:007460 bacterial DEFINED SOLID_AGAR

Overview

External ontology
nbrc.medium:114 (source link unavailable) — NBRC Medium 114
Notes
Source: NBRC Medium 114 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=114 The NBRC Medium for the 2µ Mapping Strains table lists Bacto-Yeast Nitrogen Base without amino acids, glucose, L-Leucine, L-Histidine, L-Tryptophan, L-Methionine, distilled water, and agar.

Composition

flowchart LR
    CultureMech_007460["Medium for the 2µ Mapping Strains"]
    ing0_Bacto_Yeast_Nitrogen_Base_w_o_Amino_acids__Difco_["Bacto-Yeast Nitrogen Base w/o Amino acids (Difco)"]
    CultureMech_007460 --> ing0_Bacto_Yeast_Nitrogen_Base_w_o_Amino_acids__Difco_
    ing1_Glucose["Glucose"]
    CultureMech_007460 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_L_Leucine["L-Leucine"]
    CultureMech_007460 --> ing2_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing2_L_Leucine -.-> CHEBI_15603
    ing3_L_Histidine["L-Histidine"]
    CultureMech_007460 --> ing3_L_Histidine
    CHEBI_15971["CHEBI:15971"]
    ing3_L_Histidine -.-> CHEBI_15971
    ing4_L_Tryptophan["L-Tryptophan"]
    CultureMech_007460 --> ing4_L_Tryptophan
    CHEBI_16828["CHEBI:16828"]
    ing4_L_Tryptophan -.-> CHEBI_16828
    ing5_L_Methionine["L-Methionine"]
    CultureMech_007460 --> ing5_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing5_L_Methionine -.-> CHEBI_16643
    ing6_Distilled_water["Distilled water"]
    CultureMech_007460 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377
    ing7_Agar["Agar"]
    CultureMech_007460 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Bacto-Yeast Nitrogen Base w/o Amino acids (Difco) 6.7 G_PER_L
2 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
3 L-Leucine
C6H13NO2 131.175 g/mol
60 MG_PER_L CHEBI:15603
L-leucine
4 L-Histidine
C6H9N3O2 155.157 g/mol
20 MG_PER_L CHEBI:15971
L-histidine
5 L-Tryptophan
C11H12N2O2 204.229 g/mol
20 MG_PER_L CHEBI:16828
L-tryptophan
6 L-Methionine
C5H11NO2S 149.215 g/mol
20 MG_PER_L CHEBI:16643
L-methionine
7 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
8 Agar 15 G_PER_L CHEBI:2509
agar

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:35.938584Z nbrc-import Imported from NBRC Source: NBRC, Media No. 114, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=114. Imported via ethical web scraping.
2026-03-13T03:09:32.448360Z data-quality-cleanup-v1.0 Data quality fixes Merged 7 duplicate ingredient(s)
2026-03-13T06:19:49.152818Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007460
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Bacto-Yeast Nitrogen Base w/o Amino acids (Difco)6.7gGlucose20gL-Leucine60mgL-Histidine20mgL-Tryptophan20mgL-Methionine20mgDistilled water1LAgar15g
2026-05-16T09:03:06.591026+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.624405+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 114 composition table from the preserved scrape; 8 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.782198+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 7 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-08T00:00:00-07:00 repair_nbrc_exact_score20.py RESOLVED_NBRC_EXACT_SCORE20 Resolved the recovered NBRC Medium 114 table as Medium for the 2µ Mapping Strains.