1185

CultureMech:007462 bacterial COMPLEX LIQUID

Overview

External ontology
nbrc.medium:1183 (source link unavailable) — NBRC Medium 1183
pH
7.0
Notes
Source: NBRC Medium 1183 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1183 The NBRC table lists pH 7.0, says artificial sea water is prepared from a marine aquarium salts mixture, and gives the nested trace elements recipe.

Composition

flowchart LR
    CultureMech_007462["1185"]
    ing0_KNO3["KNO3"]
    CultureMech_007462 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_Na_glycerophosphate["Na-glycerophosphate"]
    CultureMech_007462 --> ing1_Na_glycerophosphate
    ing2_Tris_HCl["Tris HCl"]
    CultureMech_007462 --> ing2_Tris_HCl
    ing3_Tryptone__Difco_0123_["Tryptone (Difco 0123)"]
    CultureMech_007462 --> ing3_Tryptone__Difco_0123_
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_007462 --> ing4_Yeast_extract
    ing5_Trace_elements__see_below_["Trace elements (see below)"]
    CultureMech_007462 --> ing5_Trace_elements__see_below_
    ing6_Artificial_sea_water["Artificial sea water"]
    CultureMech_007462 --> ing6_Artificial_sea_water

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
0.5 G_PER_L CHEBI:63043
potassium nitrate
2 Na-glycerophosphate 0.1 G_PER_L
3 Tris HCl 1 G_PER_L
4 Tryptone (Difco 0123) 2 G_PER_L MICRO:0000182 (source link unavailable)
Tryptone
5 Yeast extract 2 G_PER_L FOODON:03315426
Yeast extract
6 Trace elements (see below) 1 ML_PER_L
7 Artificial sea water 999 ML_PER_L

Solutions

Trace elements

Stock composition
IngredientConcentrationNotes
H3BO3 2.85 G_PER_L
MnCl2·4H2O 1.8 G_PER_L
FeSO4 1.36 G_PER_L
Na-tartrate 1.77 G_PER_L
CuCl2·2H2O 26.9 MG_PER_L
ZnCl2 20.8 MG_PER_L
CoCl2·6H2O 40.4 MG_PER_L
Na2MoO4·2H2O 25.2 MG_PER_L
Distilled water 1000 ML_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:36.033903Z nbrc-import Imported from NBRC Source: NBRC, Media No. 1183, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1183. Imported via ethical web scraping.
2026-03-09T08:05:10.972697+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:09:42.792990Z data-quality-cleanup-v1.0 Data quality fixes Merged 18 duplicate ingredient(s)
2026-03-13T06:19:49.159175Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007462
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-05-16T09:03:06.597954+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.648276+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 1183 composition table from the preserved scrape; 7 ingredient(s) restored, 1 stock solution(s) nested rather than flattened, 1 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.801785+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 4 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-07T00:00:00-07:00 repair_nbrc_partial_score25.py RESOLVED_NBRC_PARTIAL_SCORE25 Preserved the recovered NBRC Medium 1183 composition table.