1197

CultureMech:007463 bacterial DEFINED SOLID_AGAR

Overview

External ontology
nbrc.medium:1196 (source link unavailable) — NBRC Medium 1196
Notes
Source: NBRC Medium 1196 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1196 The NBRC Modified W-medium table lists agar as optional and describes the 10 mM substrates row as vanillic acid, syringic acid, etc.

Composition

flowchart LR
    CultureMech_007463["1197"]
    ing0_Substrates_["Substrates*"]
    CultureMech_007463 --> ing0_Substrates_
    ing1_KH2PO4["KH2PO4"]
    CultureMech_007463 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Na2HPO4_7H2O["Na2HPO4·7H2O"]
    CultureMech_007463 --> ing2_Na2HPO4_7H2O
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007463 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007463 --> ing4_MgSO4_7H2O
    ing5_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_007463 --> ing5_FeSO4_7H2O
    ing6_MgO["MgO"]
    CultureMech_007463 --> ing6_MgO
    ing7_CaCO3["CaCO3"]
    CultureMech_007463 --> ing7_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing7_CaCO3 -.-> CHEBI_3311
    ing8_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_007463 --> ing8_ZnSO4_7H2O
    ing9_MnSO4_4H2O["MnSO4·4H2O"]
    CultureMech_007463 --> ing9_MnSO4_4H2O
    ing10_CuSO4_5H2O["CuSO4·5H2O"]
    CultureMech_007463 --> ing10_CuSO4_5H2O
    ing11_CoSO4_7H2O["CoSO4·7H2O"]
    CultureMech_007463 --> ing11_CoSO4_7H2O
    ing12_H3BO4["H3BO4"]
    CultureMech_007463 --> ing12_H3BO4
    ing13_Concentrated_HCl["Concentrated HCl"]
    CultureMech_007463 --> ing13_Concentrated_HCl
    ing14_Distilled_water["Distilled water"]
    CultureMech_007463 --> ing14_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing14_Distilled_water -.-> CHEBI_15377
    ing15_Agar__if_needed_["Agar (if needed)"]
    CultureMech_007463 --> ing15_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing15_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Substrates* 10 MILLIMOLAR
2 KH2PO4
H2O4P.K 136.084 g/mol
0.85 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 Na2HPO4·7H2O 4.9 G_PER_L
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
5 MgSO4·7H2O 0.1 G_PER_L
6 FeSO4·7H2O 9.5 MG_PER_L
7 MgO 10.75 MG_PER_L
8 CaCO3
CO3.Ca 100.086 g/mol
2 MG_PER_L CHEBI:3311
calcium carbonate
9 ZnSO4·7H2O 1.44 MG_PER_L
10 MnSO4·4H2O 1.12 MG_PER_L
11 CuSO4·5H2O 0.25 MG_PER_L
12 CoSO4·7H2O 0.28 MG_PER_L
13 H3BO4 0.06 MG_PER_L
14 Concentrated HCl 0.0513 ML_PER_L
15 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
16 Agar (if needed) 15 G_PER_L CHEBI:2509
Agar

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:36.035486Z nbrc-import Imported from NBRC Source: NBRC, Media No. 1196, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1196. Imported via ethical web scraping.
2026-03-13T03:09:06.729391Z data-quality-cleanup-v1.0 Data quality fixes Merged 17 duplicate ingredient(s)
2026-03-13T06:19:49.162145Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007463
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Substrates*10mMKH2PO40.85gNa2HPO4·7H2O4.9g(NH4)2SO40.5gMgSO4·7H2O0.1gFeSO4·7H2O9.5mgMgO10.75mgCaCO32mgZnSO4·7H2O1.44mgMnSO4·4H2O1.12mgCuSO4·5H2O0.25mgCoSO4·7H2O0.28mgH3BO40.06mgConcentrated HCl51.3μlDistilled water1LAgar (if needed)15g*Vanillic acid and syringic acid etc.
2026-05-16T09:03:06.600996+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.656698+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 1196 composition table from the preserved scrape; 16 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.808276+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 4 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-07T00:00:00-07:00 repair_nbrc_partial_score25.py RESOLVED_NBRC_PARTIAL_SCORE25 Preserved the recovered NBRC Medium 1196 composition table.