1227

CultureMech:007465 bacterial COMPLEX LIQUID

Overview

External ontology
nbrc.medium:1226 (source link unavailable) — NBRC Medium 1226
Notes
Source: NBRC Medium 1226 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1226 The NBRC Medium for Strain GK12 table lists pH as unadjusted, two nested stock solutions, and aseptic anaerobic addition of the filter-sterilized bicarbonate and vitamin solutions plus sterile cysteine and sulfide solutions.

Composition

flowchart LR
    CultureMech_007465["1227"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_007465 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_007465 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007465 --> ing2_MgSO4_7H2O
    ing3_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_007465 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_NaHCO3["NaHCO3"]
    CultureMech_007465 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Glucose["Glucose"]
    CultureMech_007465 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_007465 --> ing6_Yeast_extract
    ing7_Na2S_9H2O["Na2S·9H2O"]
    CultureMech_007465 --> ing7_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_9H2O -.-> CHEBI_76209
    ing8_Cysteine_HCl["Cysteine-HCl"]
    CultureMech_007465 --> ing8_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing8_Cysteine_HCl -.-> CHEBI_91247
    ing9_Resazurin["Resazurin"]
    CultureMech_007465 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_Trace_element_solution_["Trace element solution*"]
    CultureMech_007465 --> ing10_Trace_element_solution_
    ing11_Vitamin_solution__["Vitamin solution**"]
    CultureMech_007465 --> ing11_Vitamin_solution__
    ing12_Distilled_water["Distilled water"]
    CultureMech_007465 --> ing12_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing12_Distilled_water -.-> CHEBI_15377

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.27 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4·7H2O 0.25 G_PER_L
4 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
CaCl2 x 2 H2O
5 NaHCO3
CHO3.Na 84.006 g/mol
2.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
6 Glucose
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:17234
glucose
7 Yeast extract 3 G_PER_L FOODON:03315426
Yeast extract
8 Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
30 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
9 Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
30 G_PER_L CHEBI:91247
L-cysteine hydrochloride
10 Resazurin
C12H7NO4 229.191 g/mol
1 MG_PER_L CHEBI:8806
Resazurin
11 Trace element solution* 1 ML_PER_L
12 Vitamin solution** 1 ML_PER_L
13 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water

Preparation

  1. Mix ingredients except vitamin, bicarbonate, cysteine, and sulfide solutions.
  2. Dispense the medium into culture vessels under a stream of N2/CO2 (80/20) and seal with butyl rubber stoppers.
  3. Autoclave at 121 C for 15 minutes.
  4. Filter-sterilize the bicarbonate and vitamin solutions.
  5. Aseptically and anaerobically add vitamin solution, bicarbonate solution, and cysteine and sulfide solutions using sterile syringes.

Solutions

Trace element solution

Stock composition
IngredientConcentrationNotes
Nitrilotriacetic acid (NTA) 12.8 G_PER_L
FeCl3·6H2O 1.35 G_PER_L
MnCl2·4H2O 0.1 G_PER_L
CoCl2·6H2O 24 MG_PER_L
AlCl3 13.3 MG_PER_L
ZnCl2 0.1 G_PER_L
CuCl2·2H2O 25 MG_PER_L
H3BO3 10 MG_PER_L
Na2MoO4·2H2O 24 MG_PER_L
Na2WO4·2H2O 3 MG_PER_L
NiCl2·6H2O 0.12 G_PER_L
Na2SeO3 26 MG_PER_L
Distilled water 1 L

Vitamin solution

Stock composition
IngredientConcentrationNotes
Biotin 2 MG_PER_L
Folic acid 2 MG_PER_L
Pyridoxine-HCl 10 MG_PER_L
Thiamine-HCl 5 MG_PER_L
Riboflavin 5 MG_PER_L
Nicotinic acid 5 MG_PER_L
Ca-pantothenate 5 MG_PER_L
p-Aminobenzoic acid 1 MG_PER_L
Vitamin B12 5 MG_PER_L
Lipoic acid 5 MG_PER_L
Distilled water 1 L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:36.038693Z nbrc-import Imported from NBRC Source: NBRC, Media No. 1226, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1226. Imported via ethical web scraping.
2026-03-09T08:04:29.361934+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:08:58.216843Z data-quality-cleanup-v1.0 Data quality fixes Merged 86 duplicate ingredient(s)
2026-03-13T06:19:49.167668Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007465
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-05-16T09:03:06.607135+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.674782+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 1226 composition table from the preserved scrape; 13 ingredient(s) restored, 2 stock solution(s) nested rather than flattened, 1 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.823919+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 17 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-08-26T02:12:32.861877+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-07T00:00:00-07:00 repair_nbrc_partial_score25.py RESOLVED_NBRC_PARTIAL_SCORE25 Preserved the recovered NBRC Medium 1226 composition table.