Brain Heart Infusion + 2% NaCl

CultureMech:007466 bacterial COMPLEX SOLID_AGAR

Overview

External ontology
nbrc.medium:1243 (source link unavailable) — NBRC Medium 1243
Notes
Source: NBRC Medium 1243 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1243 NBRC lists Brain Heart Infusion + 2% NaCl with 37 g Bacto Brain Heart Infusion, 20 g supplemental NaCl, 1 L distilled water, and 15 g agar if solid medium is needed. The BHI premix is preserved as a curated expanded constituent formula from the existing record.

Composition

flowchart LR
    CultureMech_007466["Brain Heart Infusion + 2% NaCl"]
    ing0_Calf_brains["Calf brains"]
    CultureMech_007466 --> ing0_Calf_brains
    ing1_Beef_heart["Beef heart"]
    CultureMech_007466 --> ing1_Beef_heart
    ing2_Proteose_peptone["Proteose peptone"]
    CultureMech_007466 --> ing2_Proteose_peptone
    ing3_Dextrose["Dextrose"]
    CultureMech_007466 --> ing3_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing3_Dextrose -.-> CHEBI_17634
    ing4_Sodium_chloride["Sodium chloride"]
    CultureMech_007466 --> ing4_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing4_Sodium_chloride -.-> CHEBI_26710
    ing5_Disodium_phosphate["Disodium phosphate"]
    CultureMech_007466 --> ing5_Disodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing5_Disodium_phosphate -.-> CHEBI_34683
    ing6_Distilled_water["Distilled water"]
    CultureMech_007466 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377
    ing7_NaCl["NaCl"]
    CultureMech_007466 --> ing7_NaCl
    ing7_NaCl -.-> CHEBI_26710
    ing8_Agar__if_needed_["Agar (if needed)"]
    CultureMech_007466 --> ing8_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Calf brains
Difco/BD Brain Heart Infusion 237500
variable VARIABLE UBERON:0000955
brain
 infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product
2 Beef heart
Difco/BD Brain Heart Infusion 237500
variable VARIABLE UBERON:0000948
heart
 infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product
3 Proteose peptone
Difco/BD Brain Heart Infusion 237500
10.0 G_PER_L MICRO:0000180 (source link unavailable)
proteose peptone
 Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product
4 Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
2.0 G_PER_L CHEBI:17634
D-glucose
 Carbon/energy source; Constituent of Brain Heart Infusion commercial product
5 Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product
6 Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
2.5 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product
7 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
 NBRC Medium 1243 lists 1 L distilled water.
8 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
 NBRC Medium 1243 supplements 37 g Bacto Brain Heart Infusion with 20 g additional NaCl.
9 Agar (if needed) 15 G_PER_L CHEBI:2509
agar
 NBRC Medium 1243 lists 15 g agar if needed.

Preparation

  1. Dissolve 37 g Bacto Brain Heart Infusion and 20 g NaCl in 1 L distilled water; add 15 g agar if solid medium is needed.
  2. Sterilize by autoclaving.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:36.042670Z nbrc-import Imported from NBRC Source: NBRC, Media No. 1243, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1243. Imported via ethical web scraping.
2026-03-13T03:09:22.545661Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:49.170539Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007466
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Bacto Brain Heart Infusion (Difco)37gNaCl20gDistilled water1LAgar (if needed)15g
2026-05-16T09:03:06.613175+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.686970+00:00 recover_nbrc_composition.py Removed corrupt rows, kept curated composition Record already held a grounded ingredient list, so the NBRC medium 1243 page was NOT used — it lists only the commercial premix. Dropped the empty-name row and 0 unparsed solution blob(s) (#299).
2026-09-11T00:00:00-07:00 repair_nbrc_1245_score15.py RESOLVED_NBRC_1245_SCORE15 Added the NBRC Medium 1243 source term and official title, kept the curated BHI premix expansion, and restored NBRC's 20 g NaCl, 1 L distilled water, and 15 g agar if needed.