1261

Overview

External ontology

nbrc.medium:1257 (source link unavailable) — NBRC Medium 1257

pH range

{'min': 6.8, 'max': 7.0}

Notes

Source: NBRC Medium 1257 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1257 The NBRC Medium for Strain SR45 table lists pH 6.8 to 7.0, four nested stock solutions, and aseptic anaerobic addition of bicarbonate and sulfide solutions after sealed-vessel autoclaving.

Composition

flowchart LR
    CultureMech_007467["1261"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_007467 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_007467 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_NaCl["NaCl"]
    CultureMech_007467 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_007467 --> ing3_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_6H2O -.-> CHEBI_86345
    ing4_KCl["KCl"]
    CultureMech_007467 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_007467 --> ing5_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_2H2O -.-> CHEBI_86158
    ing6_Na2SO4["Na2SO4"]
    CultureMech_007467 --> ing6_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing6_Na2SO4 -.-> CHEBI_32149
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_007467 --> ing7_Yeast_extract
    ing8_Resazurin["Resazurin"]
    CultureMech_007467 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_Sodium_acetate["Sodium acetate"]
    CultureMech_007467 --> ing9_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing9_Sodium_acetate -.-> CHEBI_32954
    ing10_Trace_element_mixture_["Trace element mixture*"]
    CultureMech_007467 --> ing10_Trace_element_mixture_
    ing11_Selenite_tungstate_solution__["Selenite-tungstate solution**"]
    CultureMech_007467 --> ing11_Selenite_tungstate_solution__
    ing12_Bicarbonate_solution___["Bicarbonate solution***"]
    CultureMech_007467 --> ing12_Bicarbonate_solution___
    ing13_Sulfide_solution____["Sulfide solution****"]
    CultureMech_007467 --> ing13_Sulfide_solution____
    ing14_Distilled_water["Distilled water"]
    CultureMech_007467 --> ing14_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing14_Distilled_water -.-> CHEBI_15377

Ingredients (15)

Preparation

1. Mix ingredients except for bicarbonate and sulfide solutions.
2. Dispense the medium into culture vessels under a stream of nitrogen gas and seal with butyl rubber stoppers.
3. Autoclave at 121 C for 15 minutes.
4. Aseptically and anaerobically add the remaining ingredients using sterile syringes and enclose N2/CO2 (80/20) mixed gas with the vessels.
5. Adjust pH to 6.8-7.0 using a compact pH meter.

Solutions

Target organisms

No target organisms are recorded.

Provenance