1275

Overview

External ontology

nbrc.medium:1274 (source link unavailable) — NBRC Medium 1274

pH

6.8

Notes

Source: NBRC Medium 1274 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1274 The NBRC Modified MJYS medium table lists final pH 6.8, two nested stock solutions, separate filtration of the vitamin solution, separate neutralization and autoclaving of Na2S, and tyndallization of sulfur before inoculation.

Composition

flowchart LR
    CultureMech_007468["1275"]
    ing0_NaCl["NaCl"]
    CultureMech_007468 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_007468 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_007468 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_007468 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007468 --> ing4_MgSO4_7H2O
    ing5_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_007468 --> ing5_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_6H2O -.-> CHEBI_86345
    ing6_KCl["KCl"]
    CultureMech_007468 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2·6H2O"]
    CultureMech_007468 --> ing7_Fe_NH4_2_SO4_2_6H2O
    CHEBI_76181["CHEBI:76181"]
    ing7_Fe_NH4_2_SO4_2_6H2O -.-> CHEBI_76181
    ing8_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_007468 --> ing8_Bacto_Yeast_Extract__Difco_
    ing9_Trace_elements_solution_["Trace elements solution*"]
    CultureMech_007468 --> ing9_Trace_elements_solution_
    ing10_Vitamin_solution__["Vitamin solution**"]
    CultureMech_007468 --> ing10_Vitamin_solution__
    ing11_NaHCO3["NaHCO3"]
    CultureMech_007468 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_Sulfur__powder_["Sulfur (powder)"]
    CultureMech_007468 --> ing12_Sulfur__powder_
    CHEBI_33403["CHEBI:33403"]
    ing12_Sulfur__powder_ -.-> CHEBI_33403
    ing13_Resazurin["Resazurin"]
    CultureMech_007468 --> ing13_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing13_Resazurin -.-> CHEBI_8806
    ing14_Na2S_9H2O["Na2S·9H2O"]
    CultureMech_007468 --> ing14_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_9H2O -.-> CHEBI_76209
    ing15_Distilled_water["Distilled water"]
    CultureMech_007468 --> ing15_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing15_Distilled_water -.-> CHEBI_15377

Ingredients (16)

Preparation

1. Mix ingredients except for vitamin solution, sulfur, and Na2S·9H2O.
2. Dispense the medium into culture vessels under a stream of N2/CO2 (80/20) and seal with butyl rubber stoppers.
3. Autoclave at 121 C for 15 minutes.
4. Separately neutralize and autoclave Na2S·9H2O as a 5% solution under N2.
5. Filter-sterilize the vitamin solution.
6. Sterilize sulfur by autoclaving for 60 minutes at 105 C on each of 3 successive days.
7. Before inoculation, aseptically and anaerobically add vitamin solution, sulfur, and Na2S·9H2O.

Solutions

Target organisms

No target organisms are recorded.

Provenance