1/3 Diluted LB Medium with Biphenyl

CultureMech:007469 bacterial COMPLEX SOLID_AGAR

Overview

External ontology
nbrc.medium:1286 (source link unavailable) — NBRC Medium 1286
pH
7.0
Notes
Source: NBRC Medium 1286 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1286 NBRC lists 1/3 Diluted LB Medium with Biphenyl at pH 7.0, made from Bacto tryptone, yeast extract, NaCl, distilled water, and agar. Biphenyl crystal is supplied on a petri-dish lid as a vapor phase.

Composition

flowchart LR
    CultureMech_007469["1/3 Diluted LB Medium with Biphenyl"]
    ing0_Bacto_tryptone["Bacto tryptone"]
    CultureMech_007469 --> ing0_Bacto_tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007469 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_007469 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Distilled_water["Distilled water"]
    CultureMech_007469 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377
    ing4_Agar["Agar"]
    CultureMech_007469 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_Biphenyl_crystal["Biphenyl crystal"]
    CultureMech_007469 --> ing5_Biphenyl_crystal
    CHEBI_17097["CHEBI:17097"]
    ing5_Biphenyl_crystal -.-> CHEBI_17097

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Bacto tryptone 3.3 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 NBRC Medium 1286 lists 3.3 g Bacto tryptone.
2 Yeast extract 1.6 G_PER_L FOODON:03315426
yeast extract
 NBRC Medium 1286 lists 1.6 g yeast extract.
3 NaCl
Cl.Na 58.443 g/mol
3.3 G_PER_L CHEBI:26710
sodium chloride
 NBRC Medium 1286 lists 3.3 g NaCl.
4 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
 NBRC Medium 1286 lists 1 L distilled water.
5 Agar 15 G_PER_L CHEBI:2509
agar
 NBRC Medium 1286 lists 15 g agar.
6 Biphenyl crystal
C12H10 154.212 g/mol
variable VARIABLE CHEBI:17097
biphenyl
 NBRC Medium 1286 supplies biphenyl crystal on a petri-dish lid as a vapor phase.

Preparation

  1. Dissolve Bacto tryptone, yeast extract, NaCl, and agar in 1 L distilled water.
  2. Adjust pH to 7.0.
  3. Supply biphenyl crystal on a petri-dish lid as a vapor phase.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:36.052413Z nbrc-import Imported from NBRC Source: NBRC, Media No. 1286, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1286. Imported via ethical web scraping.
2026-03-13T03:09:17.204574Z data-quality-cleanup-v1.0 Data quality fixes Merged 6 duplicate ingredient(s)
2026-03-13T06:19:49.180382Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007469
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Bacto tryptone3.3gYeast extract1.6gNaCl3.3gDistilled water1LAgar15gpH 7.0Biphenyl crystal is supplied on a lid of petri dish as vapor phase.
2026-05-16T09:03:06.625270+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.722237+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 1286 composition table from the preserved scrape; 5 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.862429+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 3 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-11T00:00:00-07:00 repair_nbrc_1287_score15.py RESOLVED_NBRC_1287_SCORE15 Added the NBRC Medium 1286 source term and official title, grounded the LB and agar formula, recorded pH 7.0, and represented the vapor-phase biphenyl crystal as a variable component.