1323

Overview

External ontology

nbrc.medium:1321 (source link unavailable) — NBRC Medium 1321

pH range

{'min': 7.0, 'max': 7.3}

Notes

Source: NBRC Medium 1321 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1321 The NBRC Medium for Strain Pf12B table lists pH 7.0 to 7.3, seven nested stock solutions, and sealed-vessel N2/CO2 autoclaving before aseptic anaerobic addition of yeast extract, bicarbonate, vitamin, thiamine, cyanocobalamin, and sulfide solutions.

Composition

flowchart LR
    CultureMech_007472["1323"]
    ing0_NaCl["NaCl"]
    CultureMech_007472 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_007472 --> ing1_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_6H2O -.-> CHEBI_86345
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_007472 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_Na2SO4["Na2SO4"]
    CultureMech_007472 --> ing3_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing3_Na2SO4 -.-> CHEBI_32149
    ing4_NH4Cl["NH4Cl"]
    CultureMech_007472 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_KH2PO4["KH2PO4"]
    CultureMech_007472 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_KCl["KCl"]
    CultureMech_007472 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_Sodium_formate["Sodium formate"]
    CultureMech_007472 --> ing7_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing7_Sodium_formate -.-> CHEBI_62965
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_007472 --> ing8_Yeast_extract
    ing9_Resazurin["Resazurin"]
    CultureMech_007472 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_Trace_element_mixture_["Trace element mixture*"]
    CultureMech_007472 --> ing10_Trace_element_mixture_
    ing11_Selenite_tungstate_solution__["Selenite-tungstate solution**"]
    CultureMech_007472 --> ing11_Selenite_tungstate_solution__
    ing12_Bicarbonate_solution__1_0_M____["Bicarbonate solution (1.0 M)***"]
    CultureMech_007472 --> ing12_Bicarbonate_solution__1_0_M____
    ing13_Vitamin_solution____["Vitamin solution****"]
    CultureMech_007472 --> ing13_Vitamin_solution____
    ing14_Thiamine_solution_____["Thiamine solution*****"]
    CultureMech_007472 --> ing14_Thiamine_solution_____
    ing15_Vitamin_B12_solution______["Vitamin B12 solution******"]
    CultureMech_007472 --> ing15_Vitamin_B12_solution______
    ing16_Sulfide_solution__1_0_M________["Sulfide solution (1.0 M)*******"]
    CultureMech_007472 --> ing16_Sulfide_solution__1_0_M________
    ing17_Distilled_water["Distilled water"]
    CultureMech_007472 --> ing17_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing17_Distilled_water -.-> CHEBI_15377

Ingredients (18)

Preparation

1. Mix ingredients except for yeast extract, bicarbonate, vitamin, thiamine, cyanocobalamin, and sulfide solutions.
2. Dispense the medium into culture vessels under a stream of N2/CO2 (80/20) and seal with butyl rubber stoppers.
3. Autoclave at 121 C for 15 minutes.
4. Aseptically and anaerobically add the remaining ingredients using sterile syringes.
5. Adjust pH to 7.0-7.3 using a compact pH meter.

Solutions

Target organisms

No target organisms are recorded.

Provenance