1401

CultureMech:007477 bacterial COMPLEX SOLID_AGAR

Overview

External ontology
nbrc.medium:1396 (source link unavailable) — NBRC Medium 1396
pH
5.6
Notes
Source: NBRC Medium 1396 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1396 The NBRC Modified Sour Dough Medium table lists maltose, yeast extract, Tween 80, Trypticase peptone, distilled water, optional agar, and final pH 5.6.

Composition

flowchart LR
    CultureMech_007477["1401"]
    ing0_Maltose["Maltose"]
    CultureMech_007477 --> ing0_Maltose
    CHEBI_17306["CHEBI:17306"]
    ing0_Maltose -.-> CHEBI_17306
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007477 --> ing1_Yeast_extract
    ing2_Tween_80["Tween 80"]
    CultureMech_007477 --> ing2_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing2_Tween_80 -.-> CHEBI_53426
    ing3_Trypticase_peptone["Trypticase peptone"]
    CultureMech_007477 --> ing3_Trypticase_peptone
    ing4_Distilled_water["Distilled water"]
    CultureMech_007477 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_Agar__if_needed_["Agar (if needed)"]
    CultureMech_007477 --> ing5_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Maltose
C12H22O11 342.297 g/mol
20 G_PER_L CHEBI:17306
Maltose
2 Yeast extract 3 G_PER_L FOODON:03315426
Yeast extract
3 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
0.3 G_PER_L CHEBI:53426
polysorbate 80
4 Trypticase peptone 6 G_PER_L MICRO:0000175 (source link unavailable)
Trypticase peptone
5 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
6 Agar (if needed) 15 G_PER_L CHEBI:2509
Agar

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:36.071743Z nbrc-import Imported from NBRC Source: NBRC, Media No. 1396, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=1396. Imported via ethical web scraping.
2026-03-13T03:09:00.869138Z data-quality-cleanup-v1.0 Data quality fixes Merged 6 duplicate ingredient(s)
2026-03-13T06:19:49.205550Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007477
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Maltose20gYeast extract3gTween 800.3gTrypticase peptone6gDistilled water1LAgar (if needed)15gpH 5.6
2026-05-16T09:03:06.657132+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.811847+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 1396 composition table from the preserved scrape; 6 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.933565+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 2 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-07T00:00:00-07:00 repair_nbrc_partial_score25.py RESOLVED_NBRC_PARTIAL_SCORE25 Preserved the recovered NBRC Medium 1396 composition table.