Bennett's Agar

CultureMech:007479 bacterial COMPLEX SOLID_AGAR

Overview

External ontology
nbrc.medium:228 (source link unavailable) — NBRC Medium 228
pH
7.3
Notes
Source: NBRC Medium 228 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=228 NBRC lists Bennett's Agar with yeast extract, beef extract, N-Z-Amine A, glucose, distilled water, and agar at final pH 7.3.

Composition

flowchart LR
    CultureMech_007479["Bennett's Agar"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_007479 --> ing0_Yeast_extract
    ing1_Beef_extract["Beef extract"]
    CultureMech_007479 --> ing1_Beef_extract
    ing2_N_Z_Amine_A["N-Z-Amine A"]
    CultureMech_007479 --> ing2_N_Z_Amine_A
    ing3_Glucose["Glucose"]
    CultureMech_007479 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Distilled_water["Distilled water"]
    CultureMech_007479 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_Agar["Agar"]
    CultureMech_007479 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Yeast extract 1 G_PER_L
2 Beef extract 1 G_PER_L
3 N-Z-Amine A 2 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:17234
glucose
5 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
6 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Dissolve ingredients in distilled water
  2. Adjust pH if needed
  3. Sterilize by autoclaving

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:35.940581Z nbrc-import Imported from NBRC Source: NBRC, Media No. 228, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=228. Imported via ethical web scraping.
2026-03-13T03:09:30.945229Z data-quality-cleanup-v1.0 Data quality fixes Merged 6 duplicate ingredient(s)
2026-03-13T06:19:49.210265Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007479
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Yeast extract1gBeef extract1gN-Z-Amine A2gGlucose10gDistilled water1LAgar20gpH 7.3
2026-05-16T09:03:06.662376+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.825114+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 228 composition table from the preserved scrape; 6 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.942799+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 3 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-11T00:00:00-07:00 repair_nbrc_229_score15.py RESOLVED_NBRC_229_SCORE15 Added the NBRC Medium 228 source term and official title, structured the final pH, added the official NBRC reference, and mirrored CHEBI terms for glucose, distilled water, and agar into MediaIngredientMech mappings.