257

Overview

External ontology

nbrc.medium:256 (source link unavailable) — NBRC Medium 256

pH range

{'min': 9.0, 'max': 9.5}

Notes

Source: NBRC Medium 256 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=256 The NBRC table instructs pH adjustment to 6.5 before autoclaving, separate Na2CO3 solution sterilization before adding it to the other autoclaved ingredients, and a final pH check of 9.0 to 9.5.

Composition

flowchart LR
    CultureMech_007481["257"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_007481 --> ing0_Yeast_extract
    ing1_Bacto_Casamino_Acids__Difco_["Bacto Casamino Acids (Difco)"]
    CultureMech_007481 --> ing1_Bacto_Casamino_Acids__Difco_
    ing2_KH2PO4["KH2PO4"]
    CultureMech_007481 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_KCl["KCl"]
    CultureMech_007481 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NH4Cl["NH4Cl"]
    CultureMech_007481 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007481 --> ing5_MgSO4_7H2O
    ing6_CaSO4_2H2O["CaSO4·2H2O"]
    CultureMech_007481 --> ing6_CaSO4_2H2O
    CHEBI_32583["CHEBI:32583"]
    ing6_CaSO4_2H2O -.-> CHEBI_32583
    ing7_Sodium_glutamate["Sodium glutamate"]
    CultureMech_007481 --> ing7_Sodium_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing7_Sodium_glutamate -.-> CHEBI_64220
    ing8_Na2CO3["Na2CO3"]
    CultureMech_007481 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_NaCl["NaCl"]
    CultureMech_007481 --> ing9_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing9_NaCl -.-> CHEBI_26710
    ing10_Distilled_water__make_up_to["Distilled water, make up to"]
    CultureMech_007481 --> ing10_Distilled_water__make_up_to
    ing11_Agar__if_needed__["Agar (if needed)*"]
    CultureMech_007481 --> ing11_Agar__if_needed__
    CHEBI_2509["CHEBI:2509"]
    ing11_Agar__if_needed__ -.-> CHEBI_2509

Ingredients (12)

Preparation

1. Dissolve agar by heating before adding NaCl.
2. Adjust pH to 6.5 before autoclaving.
3. Autoclave the non-Na2CO3 ingredients.
4. Add separately sterilized Na2CO3 solution to the other autoclaved ingredients.

Target organisms

No target organisms are recorded.

Provenance