Overview
- External ontology
- nbrc.medium:268 (source link unavailable) — NBRC Medium 268
- pH range
- {'min': 7.0, 'max': 7.4}
- Notes
- Source: NBRC Medium 268 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=268 The NBRC Inorganic Salts Starch Agar table lists ISP Medium No.4 with a nested trace salts solution and an unadjusted pH range of 7.0 to 7.4.
Composition
flowchart LR
CultureMech_007483["270"]
ing0_Soluble_starch["Soluble starch"]
CultureMech_007483 --> ing0_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing0_Soluble_starch -.-> CHEBI_28017
ing1_K2HPO4__anhydrous_base_["K2HPO4 (anhydrous base)"]
CultureMech_007483 --> ing1_K2HPO4__anhydrous_base_
ing2_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_007483 --> ing2_MgSO4_7H2O
ing3_NaCl["NaCl"]
CultureMech_007483 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_007483 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_CaCO3["CaCO3"]
CultureMech_007483 --> ing5_CaCO3
CHEBI_3311["CHEBI:3311"]
ing5_CaCO3 -.-> CHEBI_3311
ing6_Trace_salts_solution_["Trace salts solution*"]
CultureMech_007483 --> ing6_Trace_salts_solution_
ing7_Bacto_Agar__Difco_["Bacto Agar (Difco)"]
CultureMech_007483 --> ing7_Bacto_Agar__Difco_
ing8_Distilled_water["Distilled water"]
CultureMech_007483 --> ing8_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing8_Distilled_water -.-> CHEBI_15377
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Soluble starch | 10 G_PER_L |
CHEBI:28017
Starch
|
|
| 2 | K2HPO4 (anhydrous base) | 1 G_PER_L | ||
| 3 | MgSO4·7H2O | 1 G_PER_L | ||
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 6 |
CaCO3
CO3.Ca 100.086 g/mol
|
2 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 7 | Trace salts solution* | 1 ML_PER_L | ||
| 8 | Bacto Agar (Difco) | 20 G_PER_L | ||
| 9 |
Distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Solutions
Trace salts solution
| Ingredient | Concentration | Notes |
|---|---|---|
| FeSO4·7H2O | 0.1 G_PER_L | |
| MnCl2·4H2O | 0.1 G_PER_L | |
| ZnSO4·7H2O | 0.1 G_PER_L | |
| Distilled water | 100 ML_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-02-20T00:22:35.945442Z | nbrc-import | Imported from NBRC | Source: NBRC, Media No. 268, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=268. Imported via ethical web scraping. |
| 2026-03-09T08:04:15.921431+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:43.211578Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 15 duplicate ingredient(s) |
| 2026-03-13T06:19:49.220691Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007483 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-05-16T09:03:06.674088+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-08-19T21:54:22.855780+00:00 | recover_nbrc_composition.py | Recovered composition from source HTML | Re-parsed the NBRC medium 268 composition table from the preserved scrape; 9 ingredient(s) restored, 1 stock solution(s) nested rather than flattened, 1 unparsed solution blob(s) removed (#299). |
| 2026-08-20T19:01:15.965241+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 5 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-09-07T00:00:00-07:00 | repair_nbrc_partial_score25.py | RESOLVED_NBRC_PARTIAL_SCORE25 | Preserved the recovered NBRC Medium 268 composition table. |