Tryptone Yeast Extract Agar (TYG)

CultureMech:007484 bacterial COMPLEX SOLID_AGAR

Overview

External ontology
nbrc.medium:279 (source link unavailable) — NBRC Medium 279
pH
7.0
Notes
Source: NBRC Medium 279 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=279 NBRC lists Tryptone Yeast Extract Agar (TYG) with Bacto Tryptone (Difco), yeast extract, glucose, distilled water, and agar at final pH 7.0.

Composition

flowchart LR
    CultureMech_007484["Tryptone Yeast Extract Agar (TYG)"]
    ing0_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
    CultureMech_007484 --> ing0_Bacto_Tryptone__Difco_
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007484 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_007484 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Distilled_water["Distilled water"]
    CultureMech_007484 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377
    ing4_Agar["Agar"]
    CultureMech_007484 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Bacto Tryptone (Difco) 3 G_PER_L
2 Yeast extract 5 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose
4 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
5 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Dissolve ingredients in distilled water
  2. Adjust pH if needed
  3. Sterilize by autoclaving

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:35.946909Z nbrc-import Imported from NBRC Source: NBRC, Media No. 279, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=279. Imported via ethical web scraping.
2026-03-13T03:09:21.233933Z data-quality-cleanup-v1.0 Data quality fixes Merged 5 duplicate ingredient(s)
2026-03-13T06:19:49.223517Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007484
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Bacto Tryptone (Difco)3gYeast extract5gGlucose5gDistilled water1LAgar20gpH 7.0
2026-05-16T09:03:06.676783+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.863423+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 279 composition table from the preserved scrape; 5 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.970834+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 3 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-11T00:00:00-07:00 repair_nbrc_280_score15.py RESOLVED_NBRC_280_SCORE15 Added the NBRC Medium 279 source term and official title, structured the final pH, added the official NBRC reference, and mirrored CHEBI terms for glucose, distilled water, and agar into MediaIngredientMech mappings.