Overview
- External ontology
- nbrc.medium:294 (source link unavailable) — NBRC Medium 294
- pH
- 5.0
- Notes
- Source: NBRC Medium 294 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=294 The NBRC table lists 5 g Bacto Yeast Extract (Difco), 1 L distilled water, and adjustment to pH 5.0 with 1 N HCl.
Composition
flowchart LR
CultureMech_007485["296"]
ing0_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_007485 --> ing0_Bacto_Yeast_Extract__Difco_
ing1_Distilled_water["Distilled water"]
CultureMech_007485 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto Yeast Extract (Difco) | 5 G_PER_L | ||
| 2 |
Distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Preparation
- Adjust pH to 5.0 with 1 N HCl.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-02-20T00:22:35.947874Z | nbrc-import | Imported from NBRC | Source: NBRC, Media No. 294, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=294. Imported via ethical web scraping. |
| 2026-03-13T03:09:21.736201Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:49.225953Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007485 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Bacto Yeast Extract (Difco)5gDistilled water1LAdjust pH to 5.0 with 1 N HCl |
| 2026-05-16T09:03:06.679353+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-08-19T21:54:22.870672+00:00 | recover_nbrc_composition.py | Recovered composition from source HTML | Re-parsed the NBRC medium 294 composition table from the preserved scrape; 2 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299). |
| 2026-08-20T19:01:15.975284+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-09-07T00:00:00-07:00 | repair_nbrc_partial_score30.py | RESOLVED_NBRC_PARTIAL_SCORE30 | Preserved the recovered NBRC Medium 294 composition table. |