Rhamnose Salt Medium

CultureMech:007486 bacterial COMPLEX LIQUID

Overview

External ontology
nbrc.medium:309 (source link unavailable) — NBRC Medium 309
pH
7.0
Notes
Source: NBRC Medium 309 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=309 NBRC lists Rhamnose Salt Medium with K2HPO4, KH2PO4, NH4Cl, MgSO4·7H2O, NaCl, CaCl2·2H2O, FeSO4·7H2O, yeast extract, rhamnose, and distilled water at final pH 7.0.

Composition

flowchart LR
    CultureMech_007486["Rhamnose Salt Medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_007486 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_007486 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_007486 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007486 --> ing3_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_7H2O -.-> CHEBI_31795
    ing4_NaCl["NaCl"]
    CultureMech_007486 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_007486 --> ing5_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_2H2O -.-> CHEBI_86158
    ing6_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_007486 --> ing6_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_7H2O -.-> CHEBI_75836
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_007486 --> ing7_Yeast_extract
    ing8_Rhamnose["Rhamnose"]
    CultureMech_007486 --> ing8_Rhamnose
    CHEBI_26546["CHEBI:26546"]
    ing8_Rhamnose -.-> CHEBI_26546
    ing9_Distilled_water["Distilled water"]
    CultureMech_007486 --> ing9_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing9_Distilled_water -.-> CHEBI_15377

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
2.9 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
2.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
2 G_PER_L CHEBI:31206
ammonium chloride
4 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 NaCl
Cl.Na 58.443 g/mol
30 MG_PER_L CHEBI:26710
sodium chloride
6 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
3 MG_PER_L CHEBI:86158
calcium chloride dihydrate
7 FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
1 MG_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 Yeast extract 3 G_PER_L
9 Rhamnose
C6H12O5 164.157 g/mol
10 G_PER_L CHEBI:26546
rhamnose
10 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water

Preparation

  1. Dissolve ingredients in distilled water
  2. Adjust pH if needed
  3. Sterilize by autoclaving

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:35.948677Z nbrc-import Imported from NBRC Source: NBRC, Media No. 309, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=309. Imported via ethical web scraping.
2026-03-13T03:09:22.615369Z data-quality-cleanup-v1.0 Data quality fixes Merged 10 duplicate ingredient(s)
2026-03-13T06:19:49.228531Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007486
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: K2HPO42.9gKH2PO42.1gNH4Cl2gMgSO4·7H2O0.4gNaCl30mgCaCl2·2H2O3mgFeSO4·7H2O1mgYeast extract3gRhamnose10gDistilled water1LpH 7.0
2026-05-16T09:03:06.682530+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.877838+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 309 composition table from the preserved scrape; 10 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.980273+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 6 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-11T00:00:00-07:00 repair_nbrc_310_score15.py RESOLVED_NBRC_310_SCORE15 Added the NBRC Medium 309 source term and official title, structured the final pH, grounded the defined formula rows, and added the official NBRC reference.