324

CultureMech:007487 bacterial COMPLEX SOLID_AGAR

Overview

External ontology
nbrc.medium:323 (source link unavailable) — NBRC Medium 323
Notes
Source: NBRC Medium 323 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=323 The NBRC table lists equal volumes of Solution A and Solution B, Solution A pH 3.0 to 4.0, and separate autoclaving of both solutions before mixing.

Composition

flowchart LR
    CultureMech_007487["324"]
    ing0_Solution_A_["Solution A*"]
    CultureMech_007487 --> ing0_Solution_A_
    ing1_Solution_B__["Solution B**"]
    CultureMech_007487 --> ing1_Solution_B__

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Solution A* 500 ML_PER_L
2 Solution B** 500 ML_PER_L

Preparation

  1. Sterilize Solutions A and B separately by autoclaving.
  2. Mix Solution A and Solution B after separate autoclaving.

Solutions

Solution A

Stock composition
IngredientConcentrationNotes
Yeast extract 1 G_PER_L
(NH4)2SO4 0.2 G_PER_L
MgSO4·7H2O 0.5 G_PER_L
CaCl2·2H2O 0.25 G_PER_L
KH2PO4 0.6 G_PER_L
Distilled water 500 ML_PER_L

Solution B

Stock composition
IngredientConcentrationNotes
Glucose 1 G_PER_L
Agar (if needed) 20 G_PER_L
Distilled water 500 ML_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:35.949973Z nbrc-import Imported from NBRC Source: NBRC, Media No. 323, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=323. Imported via ethical web scraping.
2026-03-09T08:04:40.494438+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:09:10.483135Z data-quality-cleanup-v1.0 Data quality fixes Merged 16 duplicate ingredient(s)
2026-03-13T06:19:49.231563Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007487
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-05-16T09:03:06.685723+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.886040+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 323 composition table from the preserved scrape; 2 ingredient(s) restored, 2 stock solution(s) nested rather than flattened, 1 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.987856+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 5 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-07T00:00:00-07:00 repair_nbrc_partial_score25.py RESOLVED_NBRC_PARTIAL_SCORE25 Preserved the recovered NBRC Medium 323 composition table.