336

CultureMech:007488 bacterial COMPLEX SOLID_AGAR

Overview

External ontology
nbrc.medium:335 (source link unavailable) — NBRC Medium 335
pH
7.0
Notes
Source: NBRC Medium 335 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=335 The NBRC Medium for Marine Flexibacteria table lists final pH 7.0, a nested trace element solution, and either filtered aged seawater or Daigo's Artificial Seawater SP.

Composition

flowchart LR
    CultureMech_007488["336"]
    ing0_KNO3["KNO3"]
    CultureMech_007488 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_Sodium_glycerophosphate["Sodium glycerophosphate"]
    CultureMech_007488 --> ing1_Sodium_glycerophosphate
    ing2_Trace_element_solution_["Trace element solution*"]
    CultureMech_007488 --> ing2_Trace_element_solution_
    ing3_Tris__hydroxymethyl__aminomethane["Tris (hydroxymethyl) aminomethane"]
    CultureMech_007488 --> ing3_Tris__hydroxymethyl__aminomethane
    ing4_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
    CultureMech_007488 --> ing4_Bacto_Tryptone__Difco_
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_007488 --> ing5_Yeast_extract
    ing6_Agar__if_needed_["Agar (if needed)"]
    CultureMech_007488 --> ing6_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar__if_needed_ -.-> CHEBI_2509
    ing7_Seawater__["Seawater**"]
    CultureMech_007488 --> ing7_Seawater__

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
0.5 G_PER_L CHEBI:63043
potassium nitrate
2 Sodium glycerophosphate 0.1 G_PER_L
3 Trace element solution* 1 ML_PER_L
4 Tris (hydroxymethyl) aminomethane 1 G_PER_L
5 Bacto Tryptone (Difco) 5 G_PER_L MICRO:0000182 (source link unavailable)
Tryptone
6 Yeast extract 5 G_PER_L FOODON:03315426
Yeast extract
7 Agar (if needed) 15 G_PER_L CHEBI:2509
Agar
8 Seawater** 1 L

Solutions

Trace element solution

Stock composition
IngredientConcentrationNotes
H3BO4 2.85 G_PER_L
MnCl2·4H2O 1.8 G_PER_L
FeSO4·7H2O 1.36 G_PER_L
CuCl2·2H2O 26.9 MG_PER_L
ZnCl2 20.8 MG_PER_L
CoCl2·6H2O 40.4 MG_PER_L
Na2MoO4·2H2O 25.2 MG_PER_L
Sodium tartrate 1.77 G_PER_L
Distilled water 1 L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:35.951125Z nbrc-import Imported from NBRC Source: NBRC, Media No. 335, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=335. Imported via ethical web scraping.
2026-03-09T08:05:06.439406+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:09:38.196513Z data-quality-cleanup-v1.0 Data quality fixes Merged 23 duplicate ingredient(s)
2026-03-13T06:19:49.234684Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007488
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-05-16T09:03:06.688954+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.895233+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 335 composition table from the preserved scrape; 8 ingredient(s) restored, 1 stock solution(s) nested rather than flattened, 1 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:15.995663+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 4 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-07T00:00:00-07:00 repair_nbrc_partial_score25.py RESOLVED_NBRC_PARTIAL_SCORE25 Preserved the recovered NBRC Medium 335 composition table.