Overview
- External ontology
- nbrc.medium:352 (source link unavailable) — NBRC Medium 352
- pH
- 7.0
- Notes
- Source: NBRC Medium 352 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=352 The NBRC Methylobacterium Medium table lists final pH 7.0, Hipolypepton from Wako Pure Chemical Industries, and separate filtration of methanol.
Composition
flowchart LR
CultureMech_007489["355"]
ing0_Hipolypepton_["Hipolypepton*"]
CultureMech_007489 --> ing0_Hipolypepton_
ing1_Yeast_extract["Yeast extract"]
CultureMech_007489 --> ing1_Yeast_extract
ing2_Methanol__["Methanol**"]
CultureMech_007489 --> ing2_Methanol__
CHEBI_17790["CHEBI:17790"]
ing2_Methanol__ -.-> CHEBI_17790
ing3_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_007489 --> ing3_MgSO4_7H2O
ing4_Agar__if_needed_["Agar (if needed)"]
CultureMech_007489 --> ing4_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing4_Agar__if_needed_ -.-> CHEBI_2509
ing5_Distilled_water["Distilled water"]
CultureMech_007489 --> ing5_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing5_Distilled_water -.-> CHEBI_15377
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Hipolypepton* | 10 G_PER_L | ||
| 2 | Yeast extract | 2 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 3 |
Methanol**
CH4O 32.042 g/mol
|
5 ML_PER_L |
CHEBI:17790
Methanol
|
|
| 4 | MgSO4·7H2O | 1 G_PER_L | ||
| 5 | Agar (if needed) | 15 G_PER_L |
CHEBI:2509
Agar
|
|
| 6 |
Distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Preparation
- Sterilize methanol separately by filtration.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-02-20T00:22:35.952707Z | nbrc-import | Imported from NBRC | Source: NBRC, Media No. 352, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=352. Imported via ethical web scraping. |
| 2026-03-13T03:08:51.097452Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 10 duplicate ingredient(s) |
| 2026-03-13T06:19:49.237335Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007489 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Hipolypepton*10gYeast extract2gMethanol**5mlMgSO4·7H2O1gAgar (if needed)15gDistilled water1LpH 7.0*Wako Pure Chemical Ind., Ltd., Osaka, Japan.**Sterilize separately by filtration. |
| 2026-05-16T09:03:06.691933+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-08-19T21:54:22.905395+00:00 | recover_nbrc_composition.py | Recovered composition from source HTML | Re-parsed the NBRC medium 352 composition table from the preserved scrape; 6 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299). |
| 2026-08-20T19:01:16.006166+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-09-07T00:00:00-07:00 | repair_nbrc_partial_score25.py | RESOLVED_NBRC_PARTIAL_SCORE25 | Preserved the recovered NBRC Medium 352 composition table. |