Overview
- External ontology
- nbrc.medium:366 (source link unavailable) — NBRC Medium 366
- pH
- 5.5
- Notes
- Source: NBRC Medium 366 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=366 The NBRC table lists peptone, meat extract, yeast extract, glucose, Tween 80, phosphate, acetate, diammonium hydrogencitrate, magnesium sulfate, manganese sulfate hydrate, optional agar, and final pH 5.5.
Composition
flowchart LR
CultureMech_007490["367"]
ing0_Peptone["Peptone"]
CultureMech_007490 --> ing0_Peptone
ing1_Meat_extract["Meat extract"]
CultureMech_007490 --> ing1_Meat_extract
ing2_Yeast_extract["Yeast extract"]
CultureMech_007490 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_007490 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Tween_80["Tween 80"]
CultureMech_007490 --> ing4_Tween_80
CHEBI_53426["CHEBI:53426"]
ing4_Tween_80 -.-> CHEBI_53426
ing5_K2HPO4["K2HPO4"]
CultureMech_007490 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Sodium_acetate["Sodium acetate"]
CultureMech_007490 --> ing6_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Sodium_acetate -.-> CHEBI_32954
ing7_Diammonium_hydrogencitrate["Diammonium hydrogencitrate"]
CultureMech_007490 --> ing7_Diammonium_hydrogencitrate
ing8_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_007490 --> ing8_MgSO4_7H2O
ing9_MnSO4_nH2O["MnSO4·nH2O"]
CultureMech_007490 --> ing9_MnSO4_nH2O
ing10_Distilled_water["Distilled water"]
CultureMech_007490 --> ing10_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing10_Distilled_water -.-> CHEBI_15377
ing11_Agar__if_needed_["Agar (if needed)"]
CultureMech_007490 --> ing11_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing11_Agar__if_needed_ -.-> CHEBI_2509
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 10 G_PER_L |
MICRO:0000178 (source link unavailable)
Peptone
|
|
| 2 | Meat extract | 10 G_PER_L | ||
| 3 | Yeast extract | 5 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
5 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 | Diammonium hydrogencitrate | 2 G_PER_L | ||
| 9 | MgSO4·7H2O | 0.2 G_PER_L | ||
| 10 | MnSO4·nH2O | 0.05 G_PER_L | ||
| 11 |
Distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
|
| 12 | Agar (if needed) | 15 G_PER_L |
CHEBI:2509
Agar
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-02-20T00:22:35.953837Z | nbrc-import | Imported from NBRC | Source: NBRC, Media No. 366, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=366. Imported via ethical web scraping. |
| 2026-03-13T03:09:26.666003Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 12 duplicate ingredient(s) |
| 2026-03-13T06:19:49.239630Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007490 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Peptone10gMeat extract10gYeast extract5gGlucose20gTween 801gK2HPO42gSodium acetate5gDiammonium hydrogencitrate2gMgSO4·7H2O0.2gMnSO4·nH2O0.05gDistilled water1LAgar (if needed)15gpH 5.5 |
| 2026-05-16T09:03:06.694710+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-08-19T21:54:22.912825+00:00 | recover_nbrc_composition.py | Recovered composition from source HTML | Re-parsed the NBRC medium 366 composition table from the preserved scrape; 12 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299). |
| 2026-08-20T19:01:16.015082+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 5 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-09-07T00:00:00-07:00 | repair_nbrc_partial_score25.py | RESOLVED_NBRC_PARTIAL_SCORE25 | Preserved the recovered NBRC Medium 366 composition table. |