393

Overview

External ontology

nbrc.medium:392 (source link unavailable) — NBRC Medium 392

pH

6.9

Notes

Source: NBRC Medium 392 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=392 The NBRC Archaeoglobus Medium table lists final pH 6.9 after bicarbonate addition, anaerobic N2/CO2 preparation in sealed serum bottles, sodium sulfide reduction before use, and a nested trace element solution brought to pH 7.0 with KOH.

Composition

flowchart LR
    CultureMech_007492["393"]
    ing0_KCl["KCl"]
    CultureMech_007492 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_007492 --> ing1_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_6H2O -.-> CHEBI_86345
    ing2_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007492 --> ing2_MgSO4_7H2O
    ing3_NH4Cl["NH4Cl"]
    CultureMech_007492 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_007492 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_K2HPO4["K2HPO4"]
    CultureMech_007492 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_NaCl["NaCl"]
    CultureMech_007492 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_NaHCO3["NaHCO3"]
    CultureMech_007492 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_007492 --> ing8_Bacto_Yeast_Extract__Difco_
    ing9_Sodium_lactate["Sodium lactate"]
    CultureMech_007492 --> ing9_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing9_Sodium_lactate -.-> CHEBI_75228
    ing10_Fe_NH4_2_SO4_2_7H2O["Fe(NH4)2(SO4)2·7H2O"]
    CultureMech_007492 --> ing10_Fe_NH4_2_SO4_2_7H2O
    CHEBI_131378["CHEBI:131378"]
    ing10_Fe_NH4_2_SO4_2_7H2O -.-> CHEBI_131378
    ing11_Trace_element_solution_["Trace element solution*"]
    CultureMech_007492 --> ing11_Trace_element_solution_
    ing12_Resazurin["Resazurin"]
    CultureMech_007492 --> ing12_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Resazurin -.-> CHEBI_8806
    ing13_Na2S_9H2O["Na2S·9H2O"]
    CultureMech_007492 --> ing13_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_9H2O -.-> CHEBI_76209
    ing14_Distilled_water["Distilled water"]
    CultureMech_007492 --> ing14_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing14_Distilled_water -.-> CHEBI_15377

Ingredients (15)

Preparation

1. Dissolve ingredients except bicarbonate and sulfide.
2. Bring to a boil for a few minutes.
3. Cool quickly to room temperature while gassing with N2/CO2 (80/20).
4. Add sodium bicarbonate and adjust pH to 6.9.
5. Distribute 20 ml portions into 100 ml serum bottles under N2/CO2.
6. Seal and pressurize the bottles to 2 bar overpressure, then autoclave.
7. Before use, reduce the medium with sodium sulfide from an anaerobic sterile neutral stock solution.

Solutions

Target organisms

No target organisms are recorded.

Provenance