Overview
- External ontology
- nbrc.medium:403 (source link unavailable) — NBRC Medium 403
- pH range
- {'min': 6.5, 'max': 6.9}
- Notes
- Source: NBRC Medium 403 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=403 NBRC lists Medium 403 with K2HPO4, KH2PO4, MgSO4·7H2O, NaCl, CaCO3, yeast extract, arabinose, sodium glutamate, optional agar, and distilled water at pH 6.7 +/- 0.2.
Composition
flowchart LR
CultureMech_007493["404"]
ing0_K2HPO4["K2HPO4"]
CultureMech_007493 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_007493 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_007493 --> ing2_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_7H2O -.-> CHEBI_31795
ing3_NaCl["NaCl"]
CultureMech_007493 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCO3["CaCO3"]
CultureMech_007493 --> ing4_CaCO3
CHEBI_3311["CHEBI:3311"]
ing4_CaCO3 -.-> CHEBI_3311
ing5_Yeast_extract["Yeast extract"]
CultureMech_007493 --> ing5_Yeast_extract
ing6_Arabinose["Arabinose"]
CultureMech_007493 --> ing6_Arabinose
CHEBI_22599["CHEBI:22599"]
ing6_Arabinose -.-> CHEBI_22599
ing7_Sodium_glutamate["Sodium glutamate"]
CultureMech_007493 --> ing7_Sodium_glutamate
CHEBI_64220["CHEBI:64220"]
ing7_Sodium_glutamate -.-> CHEBI_64220
ing8_Agar__if_needed_["Agar (if needed)"]
CultureMech_007493 --> ing8_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing8_Agar__if_needed_ -.-> CHEBI_2509
ing9_Distilled_water["Distilled water"]
CultureMech_007493 --> ing9_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing9_Distilled_water -.-> CHEBI_15377
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
CaCO3
CO3.Ca 100.086 g/mol
|
3 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 6 | Yeast extract | 0.6 G_PER_L | ||
| 7 |
Arabinose
C5H10O5 150.13 g/mol
|
4 G_PER_L |
CHEBI:22599
arabinose
|
|
| 8 |
Sodium glutamate
C5H8NO4.Na 169.112 g/mol
|
6 G_PER_L |
CHEBI:64220
monosodium glutamate
|
|
| 9 | Agar (if needed) | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 10 |
Distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Preparation
- Dissolve ingredients in distilled water
- Adjust pH if needed
- Sterilize by autoclaving
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-02-20T00:22:35.959767Z | nbrc-import | Imported from NBRC | Source: NBRC, Media No. 403, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=403. Imported via ethical web scraping. |
| 2026-03-13T03:09:20.830923Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 10 duplicate ingredient(s) |
| 2026-03-13T06:19:49.248371Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007493 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: K2HPO40.5gKH2PO40.6gMgSO4·7H2O0.2gNaCl0.1gCaCO33gYeast extract0.6gArabinose4gSodium glutamate6gAgar (if needed)15gDistilled water1LpH 6.7±0.2 |
| 2026-05-16T09:03:06.703980+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-08-19T21:54:22.939384+00:00 | recover_nbrc_composition.py | Recovered composition from source HTML | Re-parsed the NBRC medium 403 composition table from the preserved scrape; 10 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299). |
| 2026-08-20T19:01:16.039626+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 6 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-09-11T00:00:00-07:00 | repair_nbrc_404_score15.py | RESOLVED_NBRC_404_SCORE15 | Added the NBRC Medium 403 source term, structured the final pH range, grounded the defined formula rows, and added the official NBRC reference. |