804

CultureMech:007494 bacterial COMPLEX SOLID_AGAR

Overview

External ontology
nbrc.medium:803 (source link unavailable) — NBRC Medium 803
pH range
{'min': 6.5, 'max': 6.8}
Notes
Source: NBRC Medium 803 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=803 The NBRC table lists Hipolypepton from Wako Pure Chemical Industries, yeast extract, glucose, lactose, Tween 80, magnesium sulfate, optional agar, and final pH 6.5 to 6.8.

Composition

flowchart LR
    CultureMech_007494["804"]
    ing0_Hipolypepton_["Hipolypepton*"]
    CultureMech_007494 --> ing0_Hipolypepton_
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007494 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_007494 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Lactose["Lactose"]
    CultureMech_007494 --> ing3_Lactose
    CHEBI_17716["CHEBI:17716"]
    ing3_Lactose -.-> CHEBI_17716
    ing4_Tween_80["Tween 80"]
    CultureMech_007494 --> ing4_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing4_Tween_80 -.-> CHEBI_53426
    ing5_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007494 --> ing5_MgSO4_7H2O
    ing6_Distilled_water["Distilled water"]
    CultureMech_007494 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377
    ing7_Agar__if_needed_["Agar (if needed)"]
    CultureMech_007494 --> ing7_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Hipolypepton* 5 G_PER_L
2 Yeast extract 5 G_PER_L FOODON:03315426
Yeast extract
3 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose
4 Lactose
C12H22O11 342.297 g/mol
5 G_PER_L CHEBI:17716
Lactose
5 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
0.5 G_PER_L CHEBI:53426
polysorbate 80
6 MgSO4·7H2O 1 G_PER_L
7 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water
8 Agar (if needed) 15 G_PER_L CHEBI:2509
Agar

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:35.961045Z nbrc-import Imported from NBRC Source: NBRC, Media No. 803, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=803. Imported via ethical web scraping.
2026-03-13T03:09:22.521153Z data-quality-cleanup-v1.0 Data quality fixes Merged 10 duplicate ingredient(s)
2026-03-13T06:19:49.250910Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007494
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Hipolypepton*5gYeast extract5gGlucose5gLactose5gTween 800.5gMgSO4·7H2O1gDistilled water1LAgar (if needed)15gpH 6.5 - 6.8*Wako Pure Chemical Ind., Ltd., Osaka, Japan.
2026-05-16T09:03:06.706715+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:22.947150+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 803 composition table from the preserved scrape; 8 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:16.045234+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 3 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-07T00:00:00-07:00 repair_nbrc_partial_score25.py RESOLVED_NBRC_PARTIAL_SCORE25 Preserved the recovered NBRC Medium 803 composition table.