825

Overview

External ontology

nbrc.medium:824 (source link unavailable) — NBRC Medium 824

Notes

Source: NBRC Medium 824 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=824 The NBRC Medium for Co-culture of Strain JT table lists vitamin, trace element, Se/W, and resazurin stock solutions and a vial culture preparation under N2/CO2 with separately filter-sterilized sulfide, cysteine, and neutralized terephthalate solutions.

Composition

flowchart LR
    CultureMech_007496["825"]
    ing0_Na_terephthalate["Na-terephthalate"]
    CultureMech_007496 --> ing0_Na_terephthalate
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007496 --> ing1_Yeast_extract
    ing2_KH2PO4["KH2PO4"]
    CultureMech_007496 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MgCl2_H2O["MgCl2·H2O"]
    CultureMech_007496 --> ing3_MgCl2_H2O
    ing4_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_007496 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_007496 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Vitamin_solution_["Vitamin solution*"]
    CultureMech_007496 --> ing6_Vitamin_solution_
    ing7_Trace_element_solution__["Trace element solution**"]
    CultureMech_007496 --> ing7_Trace_element_solution__
    ing8_Se_W_solution___["Se/W solution***"]
    CultureMech_007496 --> ing8_Se_W_solution___
    ing9_Resazurin_solution____["Resazurin solution****"]
    CultureMech_007496 --> ing9_Resazurin_solution____
    ing10_NaHCO3["NaHCO3"]
    CultureMech_007496 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Na2S_9H2O["Na2S·9H2O"]
    CultureMech_007496 --> ing11_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_9H2O -.-> CHEBI_76209
    ing12_Cysteine_HCl["Cysteine-HCl"]
    CultureMech_007496 --> ing12_Cysteine_HCl
    CHEBI_91247["CHEBI:91247"]
    ing12_Cysteine_HCl -.-> CHEBI_91247
    ing13_Distilled_water["Distilled water"]
    CultureMech_007496 --> ing13_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing13_Distilled_water -.-> CHEBI_15377

Ingredients (14)

Preparation

1. Dissolve all ingredients except Na-terephthalate, Na2S·9H2O, and cysteine-HCl up to 900 ml.
2. Flush with N2/CO2 (80/20), then autoclave.
3. Filter-sterilize Na2S·9H2O, cysteine-HCl, and neutralized 0.2 M Na-terephthalate solutions separately.
4. Add filter-sterile Na2S·9H2O, cysteine-HCl, and neutralized Na-terephthalate solutions to each vial separately.

Solutions

Target organisms

No target organisms are recorded.

Provenance