Overview
- External ontology
- nbrc.medium:847 (source link unavailable) — NBRC Medium 847
- pH range
- {'min': 4.5, 'max': 5.0}
- Notes
- Source: NBRC Medium 847 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=847 The NBRC Thermoproteus and Vulcanisaeta medium table lists separate autoclaving of yeast extract, sulfide, and sulfur before aseptic anaerobic addition and final pH adjustment to 4.5 to 5.0.
Composition
flowchart LR
CultureMech_007498["848"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_007498 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_007498 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_007498 --> ing2_MgSO4_7H2O
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_007498 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_007498 --> ing4_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing4_FeCl3_6H2O -.-> CHEBI_86254
ing5_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_007498 --> ing5_MnCl2_4H2O
ing6_Na2B4O7_10H2O["Na2B4O7·10H2O"]
CultureMech_007498 --> ing6_Na2B4O7_10H2O
CHEBI_131366["CHEBI:131366"]
ing6_Na2B4O7_10H2O -.-> CHEBI_131366
ing7_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_007498 --> ing7_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_7H2O -.-> CHEBI_32312
ing8_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_007498 --> ing8_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing8_CuCl2_2H2O -.-> CHEBI_86318
ing9_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_007498 --> ing9_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_2H2O -.-> CHEBI_75213
ing10_VOSO4_nH2O["VOSO4·nH2O"]
CultureMech_007498 --> ing10_VOSO4_nH2O
ing11_CoSO4_7H2O["CoSO4·7H2O"]
CultureMech_007498 --> ing11_CoSO4_7H2O
ing12_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_007498 --> ing12_Bacto_Yeast_Extract__Difco_
ing13_Sulfur__powder_["Sulfur (powder)"]
CultureMech_007498 --> ing13_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing13_Sulfur__powder_ -.-> CHEBI_33403
ing14_Na2S_9H2O["Na2S·9H2O"]
CultureMech_007498 --> ing14_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing14_Na2S_9H2O -.-> CHEBI_76209
ing15_Resazurin["Resazurin"]
CultureMech_007498 --> ing15_Resazurin
CHEBI_8806["CHEBI:8806"]
ing15_Resazurin -.-> CHEBI_8806
ing16_Distilled_water["Distilled water"]
CultureMech_007498 --> ing16_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing16_Distilled_water -.-> CHEBI_15377
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 | MgSO4·7H2O | 0.25 G_PER_L | ||
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
CaCl2 x 2 H2O
|
|
| 5 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.02 G_PER_L |
CHEBI:86254
FeCl3 x 6 H2O
|
|
| 6 | MnCl2·4H2O | 1.8 MG_PER_L | ||
| 7 |
Na2B4O7·10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
4.5 MG_PER_L |
CHEBI:131366
Na2B4O7 x 10 H2O
|
|
| 8 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.22 MG_PER_L |
CHEBI:32312
ZnSO4 x 7 H2O
|
|
| 9 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.05 MG_PER_L |
CHEBI:86318
CuCl2 x 2 H2O
|
|
| 10 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 MG_PER_L |
CHEBI:75213
Na2MoO4 x 2 H2O
|
|
| 11 | VOSO4·nH2O | 0.03 MG_PER_L | ||
| 12 | CoSO4·7H2O | 0.01 MG_PER_L | ||
| 13 | Bacto Yeast Extract (Difco) | 1 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 14 | Sulfur (powder) | 5 G_PER_L |
CHEBI:33403
elemental sulfur
|
|
| 15 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 16 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 MG_PER_L |
CHEBI:8806
Resazurin
|
|
| 17 |
Distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Preparation
- Mix ingredients except yeast extract, sulfur, and Na2S·9H2O.
- Autoclave under a N2 atmosphere.
- Separately autoclave yeast extract as a 10% solution and Na2S·9H2O as a 5% solution under N2.
- Sterilize sulfur by autoclaving for 60 minutes at 105 C on each of 3 successive days.
- Aseptically and anaerobically add yeast extract and sulfur to the medium.
- Before inoculation, add sterile Na2S·9H2O solution and adjust pH to 4.5-5.0 with sterile 1 N H2SO4.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-02-20T00:22:35.972225Z | nbrc-import | Imported from NBRC | Source: NBRC, Media No. 847, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=847. Imported via ethical web scraping. |
| 2026-03-09T08:04:44.713137+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:14.968322Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 34 duplicate ingredient(s) |
| 2026-03-13T06:19:49.263801Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007498 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-05-16T09:03:06.726181+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-08-19T21:54:22.996032+00:00 | recover_nbrc_composition.py | Recovered composition from source HTML | Re-parsed the NBRC medium 847 composition table from the preserved scrape; 17 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 1 unparsed solution blob(s) removed (#299). |
| 2026-08-20T19:01:16.083586+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 4 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-09-07T00:00:00-07:00 | repair_nbrc_partial_score25.py | RESOLVED_NBRC_PARTIAL_SCORE25 | Preserved the recovered NBRC Medium 847 composition table. |