GYP medium (pH 6) (CultureMech:007501)

Overview

External ontology: nbrc.medium:881 (source link unavailable) — NBRC Medium 881
pH: 6.0
Notes: Source: NBRC Medium 881 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=881 NBRC lists GYP medium (pH 6) with glucose, Bacto Yeast Extract (Difco), Hipolypepton, sodium acetate, Tween 80 solution, Salts solution, and distilled water at pH 6.0. The solid medium receives 5 g/L CaCO3 and 15 g/L agar after pH adjustment.

Composition

flowchart LR
    CultureMech_007501["GYP medium (pH 6)"]
    ing0_Glucose["Glucose"]
    CultureMech_007501 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_007501 --> ing1_Bacto_Yeast_Extract__Difco_
    ing2_Hipolypepton_["Hipolypepton*"]
    CultureMech_007501 --> ing2_Hipolypepton_
    ing3_Sodium_acetate["Sodium acetate"]
    CultureMech_007501 --> ing3_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Sodium_acetate -.-> CHEBI_32954
    ing4_Tween_80_solution__["Tween 80 solution**"]
    CultureMech_007501 --> ing4_Tween_80_solution__
    ing5_Salts_solution___["Salts solution***"]
    CultureMech_007501 --> ing5_Salts_solution___
    ing6_Distilled_water["Distilled water"]
    CultureMech_007501 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377
    ing7_CaCO3["CaCO3"]
    CultureMech_007501 --> ing7_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing7_CaCO3 -.-> CHEBI_3311
    ing8_Agar["Agar"]
    CultureMech_007501 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	Glucose C6H12O6 180.156 g/mol	10 G_PER_L	CHEBI:17234 glucose
2	Bacto Yeast Extract (Difco)	10 G_PER_L
3	Hipolypepton*	5 G_PER_L
4	Sodium acetate C2H3O2.Na 82.034 g/mol	2 G_PER_L	CHEBI:32954 sodium acetate
5	Tween 80 solution**	10 ML_PER_L
6	Salts solution***	5 ML_PER_L
7	Distilled water H2O 18.015 g/mol	1 L	CHEBI:15377 water
8	CaCO3 CO3.Ca 100.086 g/mol	5 G_PER_L	CHEBI:3311 calcium carbonate	NBRC Medium 881 adds 5 g/L CaCO3 after pH adjustment for the solid medium.
9	Agar	15 G_PER_L	CHEBI:2509 agar	NBRC Medium 881 adds 15 g/L agar after pH adjustment for the solid medium.

Preparation

Prepare the basal GYP medium with Tween 80 solution and Salts solution, then adjust the medium to pH 6.0.
For the solid medium, add 5 g/L CaCO3 and 15 g/L agar after the pH is adjusted.
Prepare the Salts solution with MgSO4·7H2O, MnSO4·4H2O, FeSO4·7H2O, NaCl, and distilled water, then add one drop of 12 N HCl to each 500 ml of the salts stock.

Solutions

Tween 80 solution

Stock composition
Ingredient	Concentration	Notes
Tween 80	50 G_PER_L
Distilled water	1 L

Salts solution

Stock composition
Ingredient	Concentration	Notes
MgSO4·7H2O	40 G_PER_L
MnSO4·4H2O	2 G_PER_L
FeSO4·7H2O	2 G_PER_L
NaCl	2 G_PER_L
Distilled water	1 L

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-02-20T00:22:35.980773Z	nbrc-import	Imported from NBRC	Source: NBRC, Media No. 881, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=881. Imported via ethical web scraping.
2026-03-09T08:04:22.034678+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T03:08:50.163479Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 22 duplicate ingredient(s)
2026-03-13T06:19:49.273182Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:007501
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-05-16T09:03:06.735797+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-08-19T21:54:23.029047+00:00	recover_nbrc_composition.py	Recovered composition from source HTML	Re-parsed the NBRC medium 881 composition table from the preserved scrape; 7 ingredient(s) restored, 2 stock solution(s) nested rather than flattened, 1 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:16.109953+00:00	apply_mim_groundings.py	Adopted MIM published grounding	Grounded 7 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-11T00:00:00-07:00	repair_nbrc_882_score15.py	RESOLVED_NBRC_882_SCORE15	Added the NBRC Medium 881 source term and official title, structured the pH, added the missing source-listed CaCO3 and agar rows for the solid medium, grounded the recovered formula and nested stock solutions, converted preparation instructions into ordered steps, and added the official NBRC reference.