904

Overview

External ontology

nbrc.medium:903 (source link unavailable) — NBRC Medium 903

pH

7.0

Notes

Source: NBRC Medium 903 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=903 The NBRC TcP Medium table lists trace-mineral and vitamin stock solutions, pH adjustment to 7.0 before N2 autoclaving, separate sterilization of the vitamin, sulfur, and sulfide additions, and aseptic anaerobic addition before inoculation.

Composition

flowchart LR
    CultureMech_007503["904"]
    ing0_NaCl["NaCl"]
    CultureMech_007503 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_007503 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_NH4Cl["NH4Cl"]
    CultureMech_007503 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_007503 --> ing3_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_6H2O -.-> CHEBI_86345
    ing4_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_007503 --> ing4_MgSO4_7H2O
    ing5_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_007503 --> ing5_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_7H2O -.-> CHEBI_75836
    ing6_K2HPO4["K2HPO4"]
    CultureMech_007503 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_NaBr["NaBr"]
    CultureMech_007503 --> ing7_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing7_NaBr -.-> CHEBI_63004
    ing8_Trace_minerals_["Trace minerals*"]
    CultureMech_007503 --> ing8_Trace_minerals_
    ing9_Vitamin_solution__["Vitamin solution**"]
    CultureMech_007503 --> ing9_Vitamin_solution__
    ing10_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_007503 --> ing10_Bacto_Yeast_Extract__Difco_
    ing11_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
    CultureMech_007503 --> ing11_Bacto_Tryptone__Difco_
    ing12_PIPES["PIPES"]
    CultureMech_007503 --> ing12_PIPES
    CHEBI_39033["CHEBI:39033"]
    ing12_PIPES -.-> CHEBI_39033
    ing13_Sulfur__powder_["Sulfur (powder)"]
    CultureMech_007503 --> ing13_Sulfur__powder_
    CHEBI_33403["CHEBI:33403"]
    ing13_Sulfur__powder_ -.-> CHEBI_33403
    ing14_Resazurin["Resazurin"]
    CultureMech_007503 --> ing14_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing14_Resazurin -.-> CHEBI_8806
    ing15_Na2S_9H2O["Na2S·9H2O"]
    CultureMech_007503 --> ing15_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing15_Na2S_9H2O -.-> CHEBI_76209
    ing16_Distilled_water["Distilled water"]
    CultureMech_007503 --> ing16_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing16_Distilled_water -.-> CHEBI_15377

Ingredients (17)

Preparation

1. Mix ingredients except vitamin solution, sulfur, and Na2S·9H2O.
2. Adjust pH to 7.0.
3. Autoclave under a N2 atmosphere.
4. Filter-sterilize the vitamin solution.
5. Sterilize sulfur by autoclaving for 60 minutes at 105 C on each of 3 successive days.
6. Neutralize Na2S·9H2O as a 5% solution and autoclave under N2.
7. Before inoculation, aseptically and anaerobically add vitamin, sulfur, and Na2S·9H2O.

Solutions

Target organisms

No target organisms are recorded.

Provenance