Hydrogen-oxidizing bacteria medium

Overview

External ontology

nbrc.medium:936 (source link unavailable) — NBRC Medium 936

Notes

Source: NBRC Medium 936 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=936 NBRC lists Hydrogen-oxidizing bacteria medium with KP buffer, MgCl2·6H2O, CaCl2·2H2O, NH4Cl, Na2S2O3, trace element and vitamin stocks, Na2CO3, and distilled water. The basal medium is dispensed under H2/CO2/O2, KP buffer is autoclaved separately under N2, vitamin solution is filter-sterilized, and inoculated vessels are pressurized to 150 kPa with H2/CO2/O2. NBRC does not list a final medium pH.

Composition

flowchart LR
    CultureMech_007506["Hydrogen-oxidizing bacteria medium"]
    ing0_KP_buffer_["KP buffer*"]
    CultureMech_007506 --> ing0_KP_buffer_
    ing1_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_007506 --> ing1_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_6H2O -.-> CHEBI_86345
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_007506 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_007506 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Na2S2O3["Na2S2O3"]
    CultureMech_007506 --> ing4_Na2S2O3
    CHEBI_132112["CHEBI:132112"]
    ing4_Na2S2O3 -.-> CHEBI_132112
    ing5_Trace_elements_solution__["Trace elements solution**"]
    CultureMech_007506 --> ing5_Trace_elements_solution__
    ing6_Vitamin_solution___["Vitamin solution***"]
    CultureMech_007506 --> ing6_Vitamin_solution___
    ing7_Na2CO3["Na2CO3"]
    CultureMech_007506 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_Distilled_water["Distilled water"]
    CultureMech_007506 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377

Ingredients (9)

Preparation

1. Mix ingredients except KP buffer and vitamin solution.
2. Dispense the medium into suitable culture vessels under a stream of H2/CO2/O2 (75/20/5) and seal with butyl rubber stoppers.
3. Separately autoclave KP buffer under an N2 atmosphere.
4. Sterilize vitamin solution by filtration.
5. Prior to inoculation, aseptically add KP buffer and vitamin solution.
6. Pressurize inoculated vessels to 150 kPa with H2/CO2/O2 (75/20/5).
7. For the trace elements solution, first dissolve NTA, adjust pH to 6.5 with NaOH, then add minerals; final pH is 7.0.

Solutions

Target organisms

No target organisms are recorded.

Provenance