948

CultureMech:007507 bacterial DEFINED SOLID_AGAR

Overview

External ontology
nbrc.medium:947 (source link unavailable) — NBRC Medium 947
Notes
Source: NBRC Medium 947 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=947 The NBRC Marine Broth with 0.5% Methanol table lists Bacto Marine Broth 2216, methanol, optional agar, unadjusted pH, and separate filtration of methanol.

Composition

flowchart LR
    CultureMech_007507["948"]
    ing0_Bacto_Marine_Broth_2216__Difco_["Bacto Marine Broth 2216 (Difco)"]
    CultureMech_007507 --> ing0_Bacto_Marine_Broth_2216__Difco_
    ing1_Methanol_["Methanol*"]
    CultureMech_007507 --> ing1_Methanol_
    CHEBI_17790["CHEBI:17790"]
    ing1_Methanol_ -.-> CHEBI_17790
    ing2_Agar__if_needed_["Agar (if needed)"]
    CultureMech_007507 --> ing2_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar__if_needed_ -.-> CHEBI_2509
    ing3_Distilled_water["Distilled water"]
    CultureMech_007507 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Bacto Marine Broth 2216 (Difco) 37.4 G_PER_L
2 Methanol*
CH4O 32.042 g/mol
5 ML_PER_L CHEBI:17790
Methanol
3 Agar (if needed) 15 G_PER_L CHEBI:2509
Agar
4 Distilled water
H2O 18.015 g/mol
1 L CHEBI:15377
water

Preparation

  1. Sterilize methanol separately by filtration.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-02-20T00:22:35.997170Z nbrc-import Imported from NBRC Source: NBRC, Media No. 947, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=947. Imported via ethical web scraping.
2026-03-13T03:08:43.445251Z data-quality-cleanup-v1.0 Data quality fixes Merged 6 duplicate ingredient(s)
2026-03-13T06:19:49.291053Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007507
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Bacto Marine Broth 2216 (Difco)37.4gMethanol*5mlAgar (if needed)15gDistilled water1LpH unadjusted*Sterilize separately by filtration.
2026-05-16T09:03:06.754368+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-08-19T21:54:23.090770+00:00 recover_nbrc_composition.py Recovered composition from source HTML Re-parsed the NBRC medium 947 composition table from the preserved scrape; 4 ingredient(s) restored, 0 stock solution(s) nested rather than flattened, 0 unparsed solution blob(s) removed (#299).
2026-08-20T19:01:16.174901+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-09-07T00:00:00-07:00 repair_nbrc_partial_score25.py RESOLVED_NBRC_PARTIAL_SCORE25 Preserved the recovered NBRC Medium 947 composition table.