Overview
- External ontology
- nbrc.medium:989 (source link unavailable) — NBRC Medium 989
- pH
- 7.0
- Notes
- Source: NBRC Medium 989 | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=989 The NBRC TYL medium table lists biotin, K, M, and C stock solutions, final pH 7.0, 115 C autoclaving for 25 minutes, and aseptic anaerobic addition of filter-sterile carbonate and cysteine solutions.
Composition
flowchart LR
CultureMech_007510["990"]
ing0_Trypticase_Peptone__BBL_["Trypticase Peptone (BBL)"]
CultureMech_007510 --> ing0_Trypticase_Peptone__BBL_
ing1_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_007510 --> ing1_Bacto_Yeast_Extract__Difco_
ing2_50__Sodium_lactate["50% Sodium lactate"]
CultureMech_007510 --> ing2_50__Sodium_lactate
ing3_Biotin_solution_["Biotin solution*"]
CultureMech_007510 --> ing3_Biotin_solution_
ing4_K_solution__["K solution**"]
CultureMech_007510 --> ing4_K_solution__
ing5_M_solution___["M solution***"]
CultureMech_007510 --> ing5_M_solution___
ing6_C_solution____["C solution****"]
CultureMech_007510 --> ing6_C_solution____
ing7_Resazurin["Resazurin"]
CultureMech_007510 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_15__Na2CO3_solution["15% Na2CO3 solution"]
CultureMech_007510 --> ing8_15__Na2CO3_solution
ing9_Cysteine_HCl["Cysteine-HCl"]
CultureMech_007510 --> ing9_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing9_Cysteine_HCl -.-> CHEBI_91247
ing10_Distilled_water["Distilled water"]
CultureMech_007510 --> ing10_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing10_Distilled_water -.-> CHEBI_15377
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase Peptone (BBL) | 2 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 2 | Bacto Yeast Extract (Difco) | 2 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 3 | 50% Sodium lactate | 25 ML_PER_L | ||
| 4 | Biotin solution* | 2 ML_PER_L | ||
| 5 | K solution** | 10 ML_PER_L | ||
| 6 | M solution*** | 2.5 ML_PER_L | ||
| 7 | C solution**** | 2.5 ML_PER_L | ||
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 MG_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 | 15% Na2CO3 solution | 20 ML_PER_L | ||
| 10 |
Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.75 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
|
| 11 |
Distilled water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Preparation
- Mix ingredients except for Na2CO3 and cysteine-HCl.
- Dispense under N2/CO2 (80/20) into culture vessels and seal with butyl rubber stoppers.
- Autoclave at 115 C for 25 minutes.
- Filter-sterilize Na2CO3 and cysteine-HCl solutions.
- Aseptically and anaerobically add filter-sterile Na2CO3 and cysteine-HCl solutions.
- Adjust pH to 7.0 with 3 N HCl.
Solutions
Biotin solution
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 5 MG_PER_L | |
| Ethanol | 100 ML_PER_L |
K solution
| Ingredient | Concentration | Notes |
|---|---|---|
| KH2PO4 | 90 G_PER_L | |
| NaCl | 90 G_PER_L | |
| (NH4)2SO4 | 90 G_PER_L | |
| Distilled water | 1 L |
M solution
| Ingredient | Concentration | Notes |
|---|---|---|
| MnCl2·4H2O | 4 G_PER_L | |
| CoCl2·6H2O | 4 G_PER_L | |
| Distilled water | 1 L |
C solution
| Ingredient | Concentration | Notes |
|---|---|---|
| CaCl2·2H2O | 10.6 G_PER_L | |
| Distilled water | 1 L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-02-20T00:22:36.001571Z | nbrc-import | Imported from NBRC | Source: NBRC, Media No. 989, URL: http://www.nbrc.nite.go.jp/NBRC2/NBRCMediumDetailServlet?NO=989. Imported via ethical web scraping. |
| 2026-03-09T08:04:22.623223+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:50.811605Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 62 duplicate ingredient(s) |
| 2026-03-13T06:19:49.298981Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007510 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-05-16T09:03:06.763030+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-08-19T21:54:23.115528+00:00 | recover_nbrc_composition.py | Recovered composition from source HTML | Re-parsed the NBRC medium 989 composition table from the preserved scrape; 11 ingredient(s) restored, 4 stock solution(s) nested rather than flattened, 1 unparsed solution blob(s) removed (#299). |
| 2026-08-20T19:01:16.194144+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 10 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-09-07T00:00:00-07:00 | repair_nbrc_partial_score25.py | RESOLVED_NBRC_PARTIAL_SCORE25 | Preserved the recovered NBRC Medium 989 composition table. |