nutrient_agar

CultureMech:007511 bacterial COMPLEX SOLID_AGAR
Original name: Nutrient Agar

Overview

External ontology
nbrc.medium:1 (source link unavailable) — NBRC Medium 1
Notes
NBRC No. 1 imported Nutrient Agar as 3.0 g/L beef extract, 5.0 g/L peptone, and 15.0 g/L agar; live JCM Medium 74 confirms the same per-liter formulation with distilled water.

Composition

flowchart LR
    CultureMech_007511["nutrient_agar"]
    ing0_Beef_extract["Beef extract"]
    CultureMech_007511 --> ing0_Beef_extract
    ing1_Peptone["Peptone"]
    CultureMech_007511 --> ing1_Peptone
    ing2_Agar["Agar"]
    CultureMech_007511 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Distilled_water["Distilled water"]
    CultureMech_007511 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Beef extract 3.0 G_PER_L FOODON:03302088
beef extract
 NBRC No. 1 and JCM Medium 74 list 3.0 g/L beef extract.
2 Peptone 5.0 G_PER_L MICRO:0000178 (source link unavailable)
peptone
 NBRC No. 1 and JCM Medium 74 list 5.0 g/L peptone.
3 Agar 15.0 G_PER_L CHEBI:2509
agar
 NBRC No. 1 and JCM Medium 74 list 15.0 g/L agar.
4 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 NBRC No. 1 and JCM Medium 74 list 1.0 L distilled water.

Preparation

  1. Dissolve all ingredients in 1000 ml distilled water
  2. Autoclave at 121°C for 20 minutes

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-24T16:10:12.690885Z nbrc-import Imported from NBRC Source: NBRC, Media No. No. 1, URL: https://www.nite.go.jp/en/nbrc/cultures/media/1.html. Imported via ethical web scraping.
2026-01-28T19:48:21.502301Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:49.302038Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007511
2026-03-17T06:30:11.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:07.617151+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nutrient Agar" to "nutrient_agar" for consistent matching
2026-06-05T07:40:29.551079+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_nbrc_nutrient_agar_score25.py RESOLVED_NBRC_NUTRIENT_AGAR_SCORE25 NBRC No. 1 imported Nutrient Agar as 3.0 g/L beef extract, 5.0 g/L peptone, and 15.0 g/L agar; live JCM Medium 74 confirms the same per-liter formulation with distilled water.