modified_growth_medium_with_23_total_salt_concentration

CultureMech:007518 bacterial COMPLEX SOLID_AGAR
Original name: Modified Growth Medium With 23% Total Salt Concentration

Overview

External ontology
TOGO:M1005 — Modified Growth Medium With 23% Total Salt Concentration
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M1005 records JCM_M956-2 Modified Growth Medium With 23% Total Salt Concentration with 233 ml/L distilled water, 2 g/L yeast extract, 10 g/L Peptone (Oxoid), 767 ml/L MDS salt water from TOGO M578, 20 g/L agar, and final adjustment to pH 7.0. JCM Medium 574 defines MDS salt water as NaCl, MgCl2 x 6H2O, MgSO4 x 7H2O, KCl, and 1 M CaCl2 solution brought to 1 L and adjusted to pH 7.5 with 1 M Tris base.

Composition

flowchart LR
    CultureMech_007518["modified_growth_medium_with_23_total_salt_concentration"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007518 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007518 --> ing1_Yeast_extract
    ing2_Agar["Agar"]
    CultureMech_007518 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Peptone__Oxoid_["Peptone (Oxoid)"]
    CultureMech_007518 --> ing3_Peptone__Oxoid_

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
233 ML_PER_L CHEBI:15377
water
 TOGO M1005 lists 233 ml/L Distilled water.
2 Yeast extract 2 G_PER_L FOODON:03315426
yeast extract
 TOGO M1005 lists 2 g/L Yeast extract.
3 Agar 20 G_PER_L CHEBI:2509
agar
 TOGO M1005 lists 20 g/L Agar.
4 Peptone (Oxoid) 10 G_PER_L MICRO:0000178 (source link unavailable)
peptone
 TOGO M1005 lists 10 g/L Peptone (Oxoid).

Preparation

  1. Mix distilled water, yeast extract, agar, Peptone (Oxoid), and MDS salt water thoroughly.
  2. Adjust TOGO M1005 to pH 7.0.

Solutions

MDS salt water

Dose: 767 ML_PER_L

Stock composition
IngredientConcentrationNotes
NaCl 240.0 G_PER_L JCM Medium 574 prints this component in its MDS salt-water stock.
MgCl2 x 6H2O 30.0 G_PER_L JCM Medium 574 prints this component in its MDS salt-water stock.
MgSO4 x 7H2O 35.0 G_PER_L JCM Medium 574 prints this component in its MDS salt-water stock.
KCl 7.0 G_PER_L JCM Medium 574 prints this component in its MDS salt-water stock.
1 M CaCl2 solution 5.0 ML_PER_L JCM Medium 574 adds 5 ml/L 1 M CaCl2 solution to MDS salt water.
Distilled water 1.0 L JCM Medium 574 prints this component in its MDS salt-water stock.
TOGO M1005 adds 767 ml/L MDS salt water from TOGO M578/JCM 574.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:53.741663Z togo-import Imported from TOGO Medium Source: JCM, ID: M1005
2026-01-28T19:48:10.272794Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:16.789383+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:49.345079Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007518
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:07.676731+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Growth Medium With 23% Total Salt Concentration" to "modified_growth_medium_with_23_total_salt_concentration" for consistent matching
2026-05-16T09:03:06.819434+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:29.616448+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m1004_m1005_score15.py RESOLVED_TOGO_M1004_M1005_SCORE15 TOGO M1005 records JCM_M956-2 Modified Growth Medium With 23% Total Salt Concentration with 233 ml/L distilled water, 2 g/L yeast extract, 10 g/L Peptone (Oxoid), 767 ml/L MDS salt water from TOGO M578, 20 g/L agar, and final adjustment to pH 7.0. JCM Medium 574 defines MDS salt water as NaCl, MgCl2 x 6H2O, MgSO4 x 7H2O, KCl, and 1 M CaCl2 solution brought to 1 L and adjusted to pH 7.5 with 1 M Tris base.