sea_salts_tyg_medium

Overview

External ontology

TOGO:M1007 — Sea Salts TYG Medium

Applications

• Microbial cultivation

pH range

{'min': 7.2, 'max': 7.5}

Notes

TOGO M1007 and JCM Medium 958 define Sea Salts TYG Medium as Sea Salts (Sigma), Wolfe's mineral elixir from M471/JCM 470, Trypticase peptone, yeast extract, glucose, resazurin, Na2CO3, and distilled water, autoclaved under an N2-CO2 gas mixture, adjusted to pH 7.2-7.5 with 5% Na2CO3 solution, and amended with 5% sodium sulfide nonahydrate and 5% L-cysteine hydrochloride hydrate solutions.

Composition

flowchart LR
    CultureMech_007520["sea_salts_tyg_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007520 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007520 --> ing1_Yeast_extract
    ing2_Resazurin["Resazurin"]
    CultureMech_007520 --> ing2_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Resazurin -.-> CHEBI_8806
    ing3_Na2CO3["Na2CO3"]
    CultureMech_007520 --> ing3_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing3_Na2CO3 -.-> CHEBI_29377
    ing4_Sea_Salts__Sigma_["Sea Salts (Sigma)"]
    CultureMech_007520 --> ing4_Sea_Salts__Sigma_
    ing5_Glucose["Glucose"]
    CultureMech_007520 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_007520 --> ing6_Trypticase_peptone__BD_BBL_
    ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007520 --> ing7_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing7_Carbon_dioxide_gas -.-> CHEBI_16526
    ing8_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007520 --> ing8_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing8_Nitrogen_gas -.-> CHEBI_17997

Ingredients (9)

Preparation

1. Mix Sea Salts, Wolfe's mineral elixir, Trypticase peptone, yeast extract, glucose, resazurin, Na2CO3, and distilled water thoroughly.
2. Autoclave the mixed base under an N2-CO2 gas mixture.
3. After cooling, adjust to pH 7.2-7.5 with 5% Na2CO3 solution that has been autoclaved and stored under N2.
4. Add 6.0 ml/L each of 5% Na2S x 9H2O solution and 5% L-Cysteine HCl H2O solution, both autoclaved and stored under N2.

Solutions

Target organisms

No target organisms are recorded.

Provenance