Overview
- External ontology
- TOGO:M102 — Alkaline Cellulose Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M102 Original source: JCM - JCM_M110 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=110 Commercial Product: MacConkey Agar Supplier: Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) Catalog: BD 212123, Sigma 05465 Source: https://microbenotes.com/macconkey-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_007544["alkaline_cellulose_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_007544 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007544 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_007544 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_007544 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_CaCl2["CaCl2"]
CultureMech_007544 --> ing4_CaCl2
CHEBI_3312["CHEBI:3312"]
ing4_CaCl2 -.-> CHEBI_3312
ing5_NH4NO3["NH4NO3"]
CultureMech_007544 --> ing5_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing5_NH4NO3 -.-> CHEBI_63038
ing6_Peptone["Peptone"]
CultureMech_007544 --> ing6_Peptone
ing7_Proteose_peptone["Proteose peptone"]
CultureMech_007544 --> ing7_Proteose_peptone
ing8_Lactose_monohydrate["Lactose monohydrate"]
CultureMech_007544 --> ing8_Lactose_monohydrate
CHEBI_17716["CHEBI:17716"]
ing8_Lactose_monohydrate -.-> CHEBI_17716
ing9_Bile_salts["Bile salts"]
CultureMech_007544 --> ing9_Bile_salts
CHEBI_22868["CHEBI:22868"]
ing9_Bile_salts -.-> CHEBI_22868
ing10_Sodium_chloride["Sodium chloride"]
CultureMech_007544 --> ing10_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing10_Sodium_chloride -.-> CHEBI_26710
ing11_Neutral_red["Neutral red"]
CultureMech_007544 --> ing11_Neutral_red
CHEBI_86370["CHEBI:86370"]
ing11_Neutral_red -.-> CHEBI_86370
ing12_Crystal_violet["Crystal violet"]
CultureMech_007544 --> ing12_Crystal_violet
CHEBI_41688["CHEBI:41688"]
ing12_Crystal_violet -.-> CHEBI_41688
ing13_Agar["Agar"]
CultureMech_007544 --> ing13_Agar
CHEBI_2509["CHEBI:2509"]
ing13_Agar -.-> CHEBI_2509
ing14_Agar["Agar"]
CultureMech_007544 --> ing14_Agar
ing14_Agar -.-> CHEBI_2509
ing15_Peptone["Peptone"]
CultureMech_007544 --> ing15_Peptone
ing16_HCl["HCl"]
CultureMech_007544 --> ing16_HCl
CHEBI_17883["CHEBI:17883"]
ing16_HCl -.-> CHEBI_17883
ing17_Na2CO3["Na2CO3"]
CultureMech_007544 --> ing17_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing17_Na2CO3 -.-> CHEBI_29377
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Defined component, Inorganic compound [Merged 2 duplicates: 900.0, 100.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 5 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.4 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
NH4NO3
H4N.NO3 80.043 g/mol
|
2 G_PER_L |
CHEBI:63038
ammonium nitrate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
Peptone
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
17.0 G_PER_L |
MICRO:0000178 (source link unavailable)
peptone
|
Pancreatic digest of gelatin, provides nitrogen and amino acids; Constituent of MacConkey Agar commercial product |
| 8 |
Proteose peptone
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
3.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Meat and casein digest, provides additional nutrients; Constituent of MacConkey Agar commercial product |
| 9 |
Lactose monohydrate
C12H22O11 342.297 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
10.0 G_PER_L |
CHEBI:17716
lactose
|
Fermentable carbohydrate for differentiation. Fermentation produces acid causing pH drop and color change; Constituent of MacConkey Agar commercial product |
| 10 |
Bile salts
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
1.5 G_PER_L |
CHEBI:22868
bile salt
|
Selective agent - inhibits gram-positive bacteria; Constituent of MacConkey Agar commercial product |
| 11 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of MacConkey Agar commercial product |
| 12 |
Neutral red
C15H16N4.HCl 288.782 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
0.03 G_PER_L |
CHEBI:86370
neutral red
|
pH indicator - turns red/pink below pH 6.8, absorbed by colonies of lactose fermenters; Constituent of MacConkey Agar commercial product |
| 13 |
Crystal violet
C25H30N3.Cl 407.989 g/mol
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
0.001 G_PER_L |
CHEBI:41688
crystal violet
|
Selective agent - inhibits gram-positive bacteria; Constituent of MacConkey Agar commercial product |
| 14 |
Agar
Multiple suppliers (BD, Sigma-Aldrich, HiMedia, Thermo) MacConkey Agar BD 212123, Sigma 05465
|
13.5 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent; Constituent of MacConkey Agar commercial product |
| 15 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 16 | Peptone | 1 G_PER_L | ||
| 17 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Solution, Inorganic compound |
| 18 |
Na2CO3
CO3.2Na 105.988 g/mol
|
6 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
Solutions
Solution A
Dose: 900 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 100 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
NaHCO3 solution
Dose: variable VARIABLE
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.769545Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M102 |
| 2026-01-28T19:48:23.421562Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: HCl; Added default concentration for ingredient: NaHCO3 solution |
| 2026-03-09T08:04:38.934487+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:08.781831Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:49.534243Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007544 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:07.878142+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkaline Cellulose Agar" to "alkaline_cellulose_agar" for consistent matching |
| 2026-05-16T09:03:07.003430+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-05-17T07:06:16.330140+00:00 | cleanup_residual_errors.py | CLEANUP_RESIDUAL_ERRORS | R01_flatten_synonym_text=2 |
| 2026-06-05T07:40:29.823120+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:20.753008+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:46.734800+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |