Overview
- External ontology
- TOGO:M1032 — M13 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1032 Original source: JCM - JCM_M979 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=979
Composition
flowchart LR
CultureMech_007547["m13_medium"]
ing0_0_1_M_Tris_HCl_buffer__pH_7_5_["0.1 M Tris-HCl buffer (pH 7.5)"]
CultureMech_007547 --> ing0_0_1_M_Tris_HCl_buffer__pH_7_5_
ing1_Distilled_water["Distilled water"]
CultureMech_007547 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Yeast_extract["Yeast extract"]
CultureMech_007547 --> ing2_Yeast_extract
ing3_Artificial_seawater["Artificial seawater"]
CultureMech_007547 --> ing3_Artificial_seawater
ing4_Glucose["Glucose"]
CultureMech_007547 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Peptone["Peptone"]
CultureMech_007547 --> ing5_Peptone
ing6_Hutner_s_basal_salts__see_below_["Hutner's basal salts (see below)"]
CultureMech_007547 --> ing6_Hutner_s_basal_salts__see_below_
ing7_Biotin["Biotin"]
CultureMech_007547 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_007547 --> ing8_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing8_Calcium_pantothenate -.-> CHEBI_31345
ing9_Folic_acid["Folic acid"]
CultureMech_007547 --> ing9_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing9_Folic_acid -.-> CHEBI_27470
ing10_Vitamin_B12["Vitamin B12"]
CultureMech_007547 --> ing10_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing10_Vitamin_B12 -.-> CHEBI_176843
ing11_Riboflavin["Riboflavin"]
CultureMech_007547 --> ing11_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing11_Riboflavin -.-> CHEBI_17015
ing12_Nicotinamide["Nicotinamide"]
CultureMech_007547 --> ing12_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing12_Nicotinamide -.-> CHEBI_17154
ing13_Thiamine_HCl_2H2O["Thiamine·HCl·2H2O"]
CultureMech_007547 --> ing13_Thiamine_HCl_2H2O
ing14_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_007547 --> ing14_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4_7H2O -.-> CHEBI_31795
ing15_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_007547 --> ing15_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_2H2O -.-> CHEBI_86158
ing16_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_007547 --> ing16_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing16_FeSO4_7H2O -.-> CHEBI_75836
ing17_Nitrilotriacetic_acid__NTA_["Nitrilotriacetic acid (NTA)"]
CultureMech_007547 --> ing17_Nitrilotriacetic_acid__NTA_
CHEBI_44557["CHEBI:44557"]
ing17_Nitrilotriacetic_acid__NTA_ -.-> CHEBI_44557
ing18__NH4_6MoO24_4H2O["(NH4)6MoO24·4H2O"]
CultureMech_007547 --> ing18__NH4_6MoO24_4H2O
ing19_KOH["KOH"]
CultureMech_007547 --> ing19_KOH
CHEBI_32035["CHEBI:32035"]
ing19_KOH -.-> CHEBI_32035
ing20_NaOH["NaOH"]
CultureMech_007547 --> ing20_NaOH
CHEBI_32145["CHEBI:32145"]
ing20_NaOH -.-> CHEBI_32145
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 0.1 M Tris-HCl buffer (pH 7.5) | 50 G_PER_L | Role: Buffer; Properties: Complex component, Defined component, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1371.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 420.0, 1.0, 950.0] |
| 3 | Yeast extract | 0.25 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 | Artificial seawater | 500 G_PER_L | Role: Mineral source, Solvating media; Properties: Defined component, Complex component, Solution | |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
0.25 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 | Peptone | 0.25 G_PER_L | ||
| 7 | Hutner's basal salts (see below) | 20 G_PER_L | ||
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 9 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 10 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 11 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 12 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
5 G_PER_L |
CHEBI:17154
nicotinamide
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 14 | Thiamine·HCl·2H2O | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 15 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.335 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
99 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Nitrilotriacetic acid (NTA)
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 19 | (NH4)6MoO24·4H2O | 9.25 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 20 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 21 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Solutions
Vitamin solution (see below)
Dose: 10 G_PER_L
Metals ''44'' (see Medium [M140])
Dose: 50 G_PER_L
Cross-reference to Medium M140
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.791875Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1032 |
| 2026-01-28T19:48:35.339569Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:58.787124+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:30.491645Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:49.565557Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007547 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:07.910059+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M13 Medium" to "m13_medium" for consistent matching |
| 2026-05-16T09:03:07.039749+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:29.861788+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:20.791941+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |