artificial_seawater_medium_with_pullulan

CultureMech:007551 bacterial COMPLEX LIQUID
Original name: Artificial Seawater Medium With Pullulan

Overview

External ontology
TOGO:M1036 — Artificial Seawater Medium With Pullulan
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1036 Original source: JCM - JCM_M983 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=983

Composition

flowchart LR
    CultureMech_007551["artificial_seawater_medium_with_pullulan"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007551 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007551 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007551 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007551 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_Resazurin["Resazurin"]
    CultureMech_007551 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_KCl["KCl"]
    CultureMech_007551 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_Pullulan["Pullulan"]
    CultureMech_007551 --> ing6_Pullulan
    CHEBI_27941["CHEBI:27941"]
    ing6_Pullulan -.-> CHEBI_27941
    ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007551 --> ing7_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing7_Carbon_dioxide_gas -.-> CHEBI_16526
    ing8_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007551 --> ing8_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing8_Nitrogen_gas -.-> CHEBI_17997
    ing9_5__Na2S_9H2O["5% Na2S・9H2O"]
    CultureMech_007551 --> ing9_5__Na2S_9H2O

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 NaCl
Cl.Na 58.443 g/mol
80 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
4.5 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
6 KCl
Cl.K 74.551 g/mol
1.5 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
7 Pullulan
(C37H62O30)n
0.5 G_PER_L CHEBI:27941
pullulan
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
8 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
9 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
10 5% Na2S・9H2O 6 G_PER_L Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

Solutions

FeCl2 solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Trace element solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Selenite--tungstate solution (see Medium [M431])

Dose: 1 G_PER_L

Cross-reference to Medium M431

5% Na2CO3 solution

Dose: 20 G_PER_L

Role: Buffer; Properties: Solution, Inorganic compound, Defined component

1% NH4Cl solution

Dose: 10 G_PER_L

Role: Nitrogen source; Properties: Defined component, Inorganic compound, Solution

1% KH2PO4 solution

Dose: 5 G_PER_L

Role: Buffer; Properties: Inorganic compound, Solution, Defined component

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:53.799243Z togo-import Imported from TOGO Medium Source: JCM, ID: M1036
2026-01-28T19:48:17.345234Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:28.722623+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 7 solution(s) from ingredients
2026-03-13T06:19:49.596558Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007551
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:02.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:07.943525+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Artificial Seawater Medium With Pullulan" to "artificial_seawater_medium_with_pullulan" for consistent matching
2026-05-16T09:03:07.061523+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:29.886753+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:20.814835+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.