Overview
- External ontology
- TOGO:M1040 — CEJFB1T Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1040 Original source: JCM - JCM_M987 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=987
Composition
flowchart LR
CultureMech_007556["cejfb1t_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007556 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_007556 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007556 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_007556 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_K2HPO4["K2HPO4"]
CultureMech_007556 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Resazurin["Resazurin"]
CultureMech_007556 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_KCl["KCl"]
CultureMech_007556 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_Sodium_acetate["Sodium acetate"]
CultureMech_007556 --> ing7_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing7_Sodium_acetate -.-> CHEBI_32954
ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007556 --> ing8_Yeast_extract__BD_Difco_
ing9_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_007556 --> ing9_Tryptone__BD_Difco_
ing10_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
CultureMech_007556 --> ing10_L_Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_H2O -.-> CHEBI_91248
ing11_KOH["KOH"]
CultureMech_007556 --> ing11_KOH
CHEBI_32035["CHEBI:32035"]
ing11_KOH -.-> CHEBI_32035
ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007556 --> ing12_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing12_Carbon_dioxide_gas -.-> CHEBI_16526
ing13_Nitrogen_gas["Nitrogen gas"]
CultureMech_007556 --> ing13_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing13_Nitrogen_gas -.-> CHEBI_17997
ing14_10__MgSO4_7H2O["10% MgSO4・7H2O"]
CultureMech_007556 --> ing14_10__MgSO4_7H2O
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
855 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.8 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.5 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 9 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 10 | Tryptone (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 11 |
L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 12 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 13 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 14 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 15 | 10% MgSO4・7H2O | 50 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Solution, Defined component |
Solutions
Trace element solution (see Medium [M439])
Dose: 1 G_PER_L
Cross-reference to Medium M439
8% NaHCO3 solution*
Dose: 25 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
1 M Glucose solution
Dose: 20 G_PER_L
Role: Carbon source; Properties: Organic compound, Solution, Defined component
10% Tryptone solution
Dose: 50 G_PER_L
Properties: Undefined component
5% Na2S・9H2O solution
Dose: 8 G_PER_L
Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.808627Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1040 |
| 2026-01-28T19:48:08.932845Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:14.382739+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:08:41.445432Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:49.635262Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007556 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:07.982955+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CEJFB1T Medium" to "cejfb1t_medium" for consistent matching |
| 2026-05-16T09:03:07.106179+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:29.925519+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.766401+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |