mpob_medium

CultureMech:007557 bacterial COMPLEX LIQUID
Original name: MPOB Medium

Overview

External ontology
TOGO:M1041 — MPOB Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1041 Original source: JCM - JCM_M988 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=988

Composition

flowchart LR
    CultureMech_007557["mpob_medium"]
    ing0_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_007557 --> ing0_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing0_Na2S_9H2O -.-> CHEBI_76209
    ing1_NaHCO3["NaHCO3"]
    CultureMech_007557 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_Distilled_water["Distilled water"]
    CultureMech_007557 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_NaCl["NaCl"]
    CultureMech_007557 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007557 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_KH2PO4["KH2PO4"]
    CultureMech_007557 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NH4Cl["NH4Cl"]
    CultureMech_007557 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_Resazurin["Resazurin"]
    CultureMech_007557 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007557 --> ing8_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2_6H2O -.-> CHEBI_86345
    ing9_Na2HPO4["Na2HPO4"]
    CultureMech_007557 --> ing9_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing9_Na2HPO4 -.-> CHEBI_34683
    ing10_Glycerol["Glycerol"]
    CultureMech_007557 --> ing10_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing10_Glycerol -.-> CHEBI_17754
    ing11_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007557 --> ing11_Yeast_extract__BD_Difco_
    ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007557 --> ing12_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing12_Carbon_dioxide_gas -.-> CHEBI_16526
    ing13_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007557 --> ing13_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing13_Nitrogen_gas -.-> CHEBI_17997

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Defined component, Inorganic compound, Simple component
2 NaHCO3
CHO3.Na 84.006 g/mol
4 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
3 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
4 NaCl
Cl.Na 58.443 g/mol
0.3 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.11 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6 KH2PO4
H2O4P.K 136.084 g/mol
0.41 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
7 NH4Cl
Cl.H4N 53.492 g/mol
0.3 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
8 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
9 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
10 Na2HPO4
HO4P.2Na 141.958 g/mol
0.53 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
11 Glycerol
C3H8O3 92.094 g/mol
2.3 G_PER_L CHEBI:17754
glycerol
 Role: Carbon source; Properties: Defined component, Simple component, Organic compound
12 Yeast extract (BD-Difco) 0.4 G_PER_L Properties: Undefined component
13 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
14 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

Trace element solution SL--10 (see Medium [M433])

Dose: 1 G_PER_L

Cross-reference to Medium M433

Selenite--tungstate solution (see Medium [M558])

Dose: 1 G_PER_L

Cross-reference to Medium M558

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:53.810993Z togo-import Imported from TOGO Medium Source: JCM, ID: M1041
2026-01-28T19:48:28.831400Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:47.788330+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:19:49.645209Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007557
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:26.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:07.992872+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MPOB Medium" to "mpob_medium" for consistent matching
2026-05-16T09:03:07.117131+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:29.936817+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:29.786154+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:46.771376+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.